Genetic reporters to detect and quantify homologous recombination in yeast.

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2022-11-28 DOI:10.1016/bs.mcb.2022.10.011

Léa Marie, Michael T Kimble, Lorraine S Symington

引用次数: 0

Abstract

Homologous recombination is a conserved process that cells use to repair damaged DNA. Many genetic assays have been developed in Saccharomyces cerevisiae to measure and characterize different types of recombination events, as well as identify proteins acting in such recombination events. Here, we describe two intrachromosomal reporters that utilize ade2 heteroalleles, whereby homologous recombination can be detected by colony color and adenine prototrophy. We detail the use of these reporters to measure recombination frequency, as well as to characterize the types of recombination events.

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检测和量化酵母中同源重组的基因报告。

同源重组是细胞用于修复受损 DNA 的一种保守过程。人们在酿酒酵母中开发了许多基因检测方法，用于测量和描述不同类型的重组事件，以及鉴定在此类重组事件中起作用的蛋白质。在这里，我们介绍了两种利用ade2异等位基因的染色体内报告基因，它们可以通过菌落颜色和腺嘌呤原位来检测同源重组。我们详细介绍了利用这些报告器测量重组频率以及描述重组事件类型的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.