Co-transfer of IncFII/IncFIB and IncFII plasmids mediated by IS26 facilitates the transmission of mcr-8.1 and tmexCD1-toprJ1.

IF 4.6 2区医学 Q1 MICROBIOLOGY

Annals of Clinical Microbiology and Antimicrobials Pub Date : 2024-02-13 DOI:10.1186/s12941-024-00676-5

Qian Wang, Meng Zhang, Yue Liu, Jinmei Li, Ran Chen, Yueling Wang, Yan Jin, Yuanyuan Bai, Zhen Song, Xinglun Lu, Changyin Wang, Yingying Hao

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引用次数: 0

Abstract

Purpose: This study aimed to characterise the whole-genome structure of two clinical Klebsiella pneumoniae strains co-harbouring mcr-8.1 and tmexCD1-toprJ1, both resistant to colistin and tigecycline.

Methods: K. pneumoniae strains TGC-02 (ST656) and TGC-05 (ST273) were isolated from urine samples of different patients hospitalised at separate times in 2021. Characterisation involved antimicrobial susceptibility testing (AST), conjugation assays, whole-genome sequencing (WGS), and bioinformatics analysis. Comparative genomic analysis was conducted on mcr-8.1-carrying and tmexCD1-toprJ1-carrying plasmids.

Results: Both K. pneumoniae isolates displayed a multidrug-resistant phenotype, exhibiting resistance or reduced susceptibility to ampicillin, ampicillin/sulbactam, cefazolin, aztreonam, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, nitrofurantoin, trimethoprim/sulfamethoxazole, apramycin, tigecycline and colistin. WGS analysis revealed that clinical strain TGC-02 carried the TmexCD1-toprJ1 gene on a 200-Kb IncFII/IncFIB-type plasmid, while mcr-8 was situated on a 146-Kb IncFII-type plasmid. In clinical strain TGC-05, TmexCD1-toprJ1 was found on a 300-Kb IncFIB/IncHI1B/IncR-type plasmid, and mcr-8 was identified on a 137-Kb IncFII/IncFIA-type plasmid. Conjugation experiments assessed the transferability of these plasmids. While transconjugants were not obtained for TGC-05 despite multiple screening with tigecycline or colistin, pTGC-02-tmex and pTGC-02-mcr8 from clinical K. pneumoniae TGC-02 demonstrated self-transferability through conjugation. Notably, the rearrangement of pTGC-02-tmex and pTGC-02-mcr8 via IS26-based homologous recombination was observed. Moreover, the conjugative and fusion plasmids of the transconjugant co-harboured the tmexCD1-toprJ1 gene cluster and mcr-8.1, potentially resulting from IS26-based homologous recombination.

Conclusion: The emergence of colistin- and tigecycline-resistant K. pneumoniae strains is concerning, and effective surveillance measures should be implemented to prevent further dissemination.

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由 IS26 介导的 IncFII/IncFIB 和 IncFII 质粒的共同转移促进了 mcr-8.1 和 tmexCD1-toprJ1 的传播。

目的：本研究旨在确定两株临床肺炎克雷伯菌株的全基因组结构特征，这两株菌株共同携带 mcr-8.1 和 tmexCD1-toprJ1，均对可乐定和替加环素耐药：肺炎双球菌菌株TGC-02（ST656）和TGC-05（ST273）是从2021年不同时间住院的不同患者的尿液样本中分离出来的。特征描述包括抗菌药物敏感性测试（AST）、共轭测定、全基因组测序（WGS）和生物信息学分析。对携带 mcr-8.1 的质粒和携带 tmexCD1-toprJ1 的质粒进行了比较基因组分析：结果：两株肺炎克氏菌均表现出多重耐药表型，对氨苄西林、氨苄西林/舒巴坦、头孢唑啉、阿曲南、阿米卡星、庆大霉素、妥布霉素、环丙沙星、左氧氟沙星、硝基呋喃妥因、三甲双氨/磺胺甲噁唑、阿普霉素、替加环素和可乐定耐药或敏感性降低。WGS分析显示，临床菌株TGC-02的TmexCD1-toprJ1基因携带在一个200Kb的IncFII/IncFIB型质粒上，而mcr-8则位于一个146Kb的IncFII型质粒上。在临床菌株 TGC-05 中，TmexCD1-toprJ1 位于 300-Kb IncFIB/IncHI1B/IncR 型质粒上，而 mcr-8 则位于 137-Kb IncFII/IncFIA 型质粒上。共轭实验评估了这些质粒的可转移性。尽管使用替加环素或可乐定进行了多次筛选，但 TGC-05 仍未获得转导结合体，而来自临床肺炎 K. pneumoniae TGC-02 的 pTGC-02-tmex 和 pTGC-02-mcr8 则通过共轭证明了自身的可转移性。值得注意的是，pTGC-02-tmex 和 pTGC-02-mcr8 通过基于 IS26 的同源重组进行了重排。此外，转共轭物的共轭质粒和融合质粒中共同含有 tmexCD1-toprJ1 基因簇和 mcr-8.1，这可能是基于 IS26 的同源重组的结果：结论：耐秋水仙碱和替加环素肺炎克氏菌菌株的出现令人担忧，应采取有效的监控措施防止其进一步扩散。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Microbiology and Antimicrobials MICROBIOLOGY-

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.