Quantitative detection of Staphylococcus aureus using aptamer-based bioassay coupled with porous Si SERS platform

IF 6.7 3区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Journal of Science: Advanced Materials and Devices Pub Date : 2024-02-06 DOI:10.1016/j.jsamd.2024.100690

Divagar Muthukumar, Giorgi Shtenberg

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引用次数: 0

Abstract

The current study presents a highly sensitive and selective surface-enhanced Raman scattering (SERS) based method for detecting Staphylococcus aureus (S. aureus) in food and water samples. The bioassay includes target bacteria recognition by a specific aptamer complex, fast syringe filtration and the assessment of unbound biorecognition complexes using a silver-coated porous silicon SERS platform. Notably, the developed bioassay showed high sensitivity toward S. aureus detection, achieving an impressive detection limit of 2 CFU mL⁻¹ and a broad linear response of 10¹–10⁶ CFU mL⁻¹. Furthermore, the selectivity, regeneration and overall shelf-life were thoroughly evaluated while depicting satisfactory performances. Finally, the practicality of the developed SERS bioassay was elucidated in various samples (i.e., fish, milk, groundwater and tahini) spiked with different S. aureus concentrations that revealed recovery values of 93–110%. The successful validation of actual samples' analysis emphasizes the platform's reliability, robustness and suitability for practical use, including on-site operation.

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利用基于适配体的生物分析法和多孔硅 SERS 平台定量检测金黄色葡萄球菌

目前的研究提出了一种基于表面增强拉曼散射（SERS）的高灵敏度和高选择性方法，用于检测食品和水样品中的金黄色葡萄球菌（S. aureus）。该生物测定包括通过特异性适配体复合物识别目标细菌、快速注射器过滤以及使用银涂层多孔硅 SERS 平台评估未结合的生物识别复合物。值得注意的是，所开发的生物测定对金黄色葡萄球菌的检测显示出很高的灵敏度，达到了令人印象深刻的 2 CFU mL-1 检测限和 101-106 CFU mL-1 的宽线性响应。此外，还对选择性、再生性和总体保存期进行了全面评估，结果令人满意。最后，在添加了不同浓度金黄色葡萄球菌的各种样品（如鱼、牛奶、地下水和芝麻酱）中阐明了所开发的 SERS 生物分析法的实用性，结果显示回收率为 93-110%。实际样品分析的成功验证强调了该平台的可靠性、稳健性和实用性，包括现场操作。

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来源期刊

Journal of Science: Advanced Materials and Devices Materials Science-Electronic, Optical and Magnetic Materials

CiteScore

11.90

自引率

2.50%

发文量

审稿时长

47 days

期刊介绍： In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research. Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science. With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.