Effects of temperature acclimation on the upper thermal tolerance of two Arctic fishes.

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Conservation Physiology Pub Date : 2024-02-10 eCollection Date: 2024-01-01 DOI:10.1093/conphys/coae001
Carolyn R Waterbury, Trent M Sutton, Amanda L Kelley, J Andrés López
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引用次数: 0

Abstract

The thermally dynamic nearshore Beaufort Sea, Alaska, is experiencing climate change-driven temperature increases. Measuring thermal tolerance of broad whitefish (Coregonus nasus) and saffron cod (Eleginus gracilis), both important species in the Arctic ecosystem, will enhance understanding of species-specific thermal tolerances. The objectives of this study were to determine the extent that acclimating broad whitefish and saffron cod to 5°C and 15°C changed their critical thermal maximum (CTmax) and HSP70 protein and mRNA expression in brain, muscle and liver tissues. After acclimation to 5°C and 15°C, the species were exposed to a thermal ramping rate of 3.4°C · h-1 before quantifying the CTmax and HSP70 protein and transcript concentrations. Broad whitefish and saffron cod acclimated to 15°C had a significantly higher mean CTmax (27.3°C and 25.9°C, respectively) than 5°C-acclimated fish (23.7°C and 23.2°C, respectively), which is consistent with trends in CTmax between higher and lower acclimation temperatures. There were species-specific differences in thermal tolerance with 15°C-acclimated broad whitefish having higher CTmax and HSP70 protein concentrations in liver and muscle tissues than saffron cod at both acclimation temperatures. Tissue-specific differences were quantified, with brain and muscle tissues having the highest and lowest HSP70 protein concentrations, respectively, for both species and acclimation temperatures. The differences in broad whitefish CTmax between the two acclimation temperatures could be explained with brain and liver tissues from 15°C acclimation having higher HSP70a-201 and HSP70b-201 transcript concentrations than control fish that remained in lab-acclimation conditions of 8°C. The shift in CTmax and HSP70 protein and paralogous transcripts demonstrate the physiological plasticity that both species possess in responding to two different acclimation temperatures. This response is imperative to understand as aquatic temperatures continue to elevate.

温度适应对两种北极鱼类上层热耐受性的影响。
阿拉斯加热动态近岸波弗特海正在经历气候变化导致的温度上升。宽吻鳕(Coregonus nasus)和红花鳕(Eleginus gracilis)都是北极生态系统中的重要物种,测量这两种鱼的热耐受性将加深对物种特定热耐受性的了解。本研究的目的是确定将宽吻鳕和红花鳕分别驯化到5°C和15°C对其临界最大热量(CTmax)以及脑、肌肉和肝组织中HSP70蛋白和mRNA表达的改变程度。在适应 5°C 和 15°C 后,将鱼种暴露在 3.4°C - h-1 的热升温速率下,然后量化 CTmax 和 HSP70 蛋白及转录本的浓度。适应15°C的宽白鲑和红花鳕的平均CTmax(分别为27.3°C和25.9°C)明显高于适应5°C的鱼类(分别为23.7°C和23.2°C),这与较高和较低适应温度下CTmax的变化趋势一致。物种对热的耐受性存在差异,15°C驯化的宽白鲑在两种驯化温度下的肝脏和肌肉组织中的CTmax和HSP70蛋白浓度均高于红花鳕。对特定组织的差异进行了量化,在两个物种和适应温度下,脑组织和肌肉组织的 HSP70 蛋白浓度分别最高和最低。15°C驯化条件下的脑组织和肝脏组织的HSP70a-201和HSP70b-201转录本浓度高于8°C实验室驯化条件下的对照鱼,这可以解释两种驯化温度下宽白鲑CTmax的差异。CTmax和HSP70蛋白及旁系转录本的变化表明,这两种鱼类在对两种不同的驯化温度做出反应时具有生理可塑性。随着水生温度的不断升高,必须了解这种反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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