Phenotype Compensation in Reproductive ADAM Gene Family: A Case Study with ADAM27 Knockout Mouse.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Iranian Journal of Biotechnology Pub Date : 2022-10-01 DOI:10.30498/ijb.2022.250175.2902

Jafar Khezri, Ehsan Hasheni, Mehdi Shams Ara, Aidin Rahim Tayefe, Farid Heidari

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引用次数: 0

Abstract

Background: A disintegrin and metalloproteinase (ADAM) cell surface proteins are expressed in different cells and are involved in biological processes such as cell-cell interactions, cell differentiation, sperm attachment and fertilization. A significant number of ADAM isoforms are expressed in the reproductive tracts of male mice and other mammals, which shows the importance of this gene family in reproduction.

Objectives: The role of ADAM27 protein in reproduction was investigated.

Materials and methods: ADAM27 knock-out mutant mice were generated using the blastocyst microinjection technique. The knock-out mice were analyzed genetically and phenotypically to discover any abnormalities.

Results: The results of this study revealed that the homozygote mutant male mice were fertile and showed no significant differences compared to wild-type male mice. A histological exam, sperm analysis and in-vitro fertilization experiments showed no statistical differences.

Conclusions: We can conclude that the role of deficient ADAM27 protein is probably compensated mainly by other ADAM isoforms which are expressed in the reproductive system.

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生殖 ADAM 基因家族的表型补偿：ADAM27 基因敲除小鼠的案例研究。

背景：ADAM细胞表面蛋白在不同细胞中表达，参与细胞-细胞相互作用、细胞分化、精子附着和受精等生物学过程。雄性小鼠和其他哺乳动物的生殖道中表达大量的 ADAM 同工酶，这表明该基因家族在生殖过程中的重要性：目的：研究ADAM27蛋白在生殖过程中的作用：材料和方法：利用囊胚显微注射技术产生 ADAM27 基因敲除突变小鼠。对基因敲除小鼠进行基因和表型分析，以发现任何异常：研究结果表明，同基因突变雄性小鼠具有生育能力，与野生型雄性小鼠相比没有明显差异。组织学检查、精子分析和体外受精实验均未发现统计学差异：我们可以得出结论，ADAM27 蛋白缺陷的作用可能主要由在生殖系统中表达的其他 ADAM 异构体来弥补。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

7.70%

发文量

期刊介绍： Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.