SOX2 Overlapping Transcript (SOX2-OT) Enhances the Lung Cancer Malignancy Through Interaction with miR-194-5p/SOX5 Axis.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Iranian Journal of Biotechnology Pub Date : 2023-07-01 DOI:10.30498/ijb.2023.364919.3530

Zahra Sadeghi, Fatemeh Dodangeh, Jamshid Raheb

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引用次数: 0

Abstract

Background: Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.

Objectives: This study aimed to show that SOX2-OT as a long non-coding RNA (IncRNA) regulates gene expression via the SOX2-OT/miR-194-5p/SOX5 axis molecular pathway in lung cancer.

Materials and methods: A549 cells transfected with siRNA-SOX2-OT and the expression of SOX2-OT and miR-194-5p genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9, phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated by Western blotting.

Results: After using siRNA-SOX2-OT, an increase in the expression of miR-194-5p and a decrease in the expression of B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.

Conclusions: According to the results of the present study, an increase in SOX2-OT in lung cancer seems to stimulate the expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.

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SOX2重叠转录本（SOX2-OT）通过与miR-194-5p/SOX5轴相互作用增强肺癌恶性程度

背景：肺癌是最常见的癌症类型之一，也是全球癌症相关死亡的主要原因。因此，了解与肺癌恶性程度相关的生物标志物非常有用：本研究旨在证明 SOX2-OT 作为一种长非编码 RNA（IncRNA）通过 SOX2-OT/miR-194-5p/SOX5 轴分子通路调控肺癌中的基因表达：用siRNA-SOX2-OT转染A549细胞，转染前后实时PCR分析SOX2-OT和miR-194-5p基因的表达。此外，转染前后 B-catenin、MMP9、磷酸化和活化的 STAT3（p-STAT3）、SOX5 和 VEGF 蛋白的表达情况也通过 Western 印迹法进行了检测：结果：使用 siRNA-SOX2-OT 后，观察到 miR-194-5p 表达增加，B-catenin、SOX5、p-STAT3 活化的 STAT3、VEGF 和 MMP9 蛋白表达减少：结论：根据本研究的结果，肺癌中 SOX2-OT 的增加似乎会刺激 beta-catenin、SOX5、MMP9 和 VEGF 的表达，从而支持肺癌细胞的恶性程度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

7.70%

发文量

期刊介绍： Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.