Evaluation of Surface Properties of Two Remineralizing Agents after Modification by Chitosan Nano Particles: An In vitro Study.

IF 0.9 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Contemporary Clinical Dentistry Pub Date : 2023-10-01 Epub Date: 2023-12-19 DOI:10.4103/ccd.ccd_84_23

Dina Mohamed Wahied, Naglaa Ezzeldin, Ali Abdelnabi, Maha Sherif Othman, Mai Hesham Abd El Rahman

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引用次数: 0

Abstract

Background: Calcium phosphate-based systems have been introduced as promising bio-mimetic materials due to their close resemblance to the enamel. Chitosan and its derivatives have been an emerging biomaterial due to their additional antibacterial effect and promising re-mineralizing ability.

Aim: The aim of this study is to evaluate the effect of chitosan nanoparticles on the remineralization of the demineralized enamel surface after being added to nano-hydroxyapatite and nano-calcium phosphate materials.

Setting and design: This was in vitro study.

Materials and methods: Twenty specimens of extracted permanent molars were collected, and then immersed in demineralizing solution, then distributed into four groups according to the remineralizing material. Group 1: Treated with Nano-beta-tricalcium phosphate (Nβ-TCP) gel, Group 2: Treated with Nβ-TCP with chitosan gel, Group 3: Treated with Nanohydroxyapatite (NHA) gel, and Group 4: Treated with NHA with chitosan gel. The surface hardness of the teeth was measured at baseline, after demineralization, and after remineralization. The structural changes in each group were analyzed using the scanning electron microscopy.

Statistical analysis: Shapiro-Wilk's test, one-way ANOVA followed by Tukey's post hoc test was used.

Results: In all groups, there was a significant difference in mean Vickers hardness number (VHN) at different intervals, with the highest value found after treatment (301.64-395.65) VHN, followed by the baseline (236.97-276.15) VHN, while the lowest value was detected after demineralization (121.23-124.39) VHN. It was also indicated that baseline treatment, the Hardness percentage change (%) of the nano NHA + Chitosan group showed the highest significant value (55.10%), while the Nβ-TCP group exhibited the lowest significant value (9.56%).

Conclusions: It can be concluded that NHA and NΒ-TCP modified by chitosan NPs as remineralizing agents of enamel surface hold promising results.

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壳聚糖纳米颗粒修饰后两种再矿化剂表面性质的评估：体外研究

背景：以磷酸钙为基础的系统因其与牙釉质非常相似而被认为是一种有前景的生物仿生材料。目的：本研究旨在评估壳聚糖纳米颗粒加入纳米羟基磷灰石和纳米磷酸钙材料后对脱矿釉质表面再矿化的影响：这是一项体外研究：收集20颗拔出的恒磨牙标本，将其浸泡在脱矿溶液中，然后根据再矿化材料的不同分为四组。第一组：使用纳米β-磷酸三钙（Nβ-TCP）凝胶；第二组：使用Nβ-TCP加壳聚糖凝胶；第三组：使用纳米羟基磷灰石（NHA）凝胶；第四组：使用NHA加壳聚糖凝胶。分别在基线、脱矿和再矿化后测量牙齿的表面硬度。使用扫描电子显微镜分析各组的结构变化：采用 Shapiro-Wilk 检验、单因素方差分析和 Tukey 后检验：在所有组别中，不同时间段的平均维氏硬度数（VHN）存在显著差异，治疗后的维氏硬度数（301.64-395.65）最高，其次是基线（236.97-276.15），而脱矿后的维氏硬度数（121.23-124.39）最低。研究还表明，在基线处理中，纳米 NHA + 壳聚糖组的硬度百分比变化（%）显示出最高的显著值（55.10%），而 Nβ-TCP 组则显示出最低的显著值（9.56%）：结论：壳聚糖 NPs 修饰的 NHA 和 NΒ-TCP 作为珐琅质表面的再矿化剂具有良好的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Contemporary Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： The journal Contemporary Clinical Dentistry (CCD) (Print ISSN: 0976-237X, E-ISSN:0976- 2361) is peer-reviewed journal published on behalf of Maharishi Markandeshwar University and issues are published quarterly in the last week of March, June, September and December. The Journal publishes Original research papers, clinical studies, case series strictly of clinical interest. Manuscripts are invited from all specialties of Dentistry i.e. Conservative dentistry and Endodontics, Dentofacial orthopedics and Orthodontics, Oral medicine and Radiology, Oral pathology, Oral surgery, Orodental diseases, Pediatric Dentistry, Periodontics, Clinical aspects of Public Health dentistry and Prosthodontics. Review articles are not accepted. Review, if published, will only be by invitation from eminent scholars and academicians of National and International repute in the field of Medical/Dental education.