All-trans retinoic acid alleviates transmissible gastroenteritis virus-induced intestinal inflammation and barrier dysfunction in weaned piglets.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Junning Pu, Daiwen Chen, Gang Tian, Jun He, Ping Zheng, Zhiqing Huang, Xiangbing Mao, Jie Yu, Yuheng Luo, Junqiu Luo, Hui Yan, Aimin Wu, Bing Yu
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Abstract

Background: Transmissible gastroenteritis virus (TGEV) is one of the main pathogens causing severe diarrhea of piglets. The pathogenesis of TGEV is closely related to intestinal inflammation. All-trans retinoic acid (ATRA) is the main active metabolite of vitamin A, which has immunomodulatory and anti-inflammatory properties. However, it is unclear whether ATRA can alleviate TGEV-induced intestinal inflammation and barrier dysfunction in piglets. This study aimed to investigate the effects of ATRA on growth performance, diarrhea, intestinal inflammation and intestinal barrier integrity of TGEV-challenged piglets.

Methods: In a 19-d study, 32 weaned piglets were randomly divided into 4 treatments: Control group (basal diet), TGEV group (basal diet + TGEV challenge), TGEV + ATRA5 group (basal diet + 5 mg/d ATRA + TGEV challenge) and TGEV + ATRA15 group (basal diet + 15 mg/d ATRA + TGEV challenge). On d 14, piglets were orally administered TGEV or the sterile medium.

Results: Feeding piglets with 5 and 15 mg/d ATRA alleviated the growth inhibition and diarrhea induced by TGEV (P < 0.05). Feeding piglets with 5 and 15 mg/d ATRA also inhibited the increase of serum diamine oxidase (DAO) activity and the decrease of occludin and claudin-1 protein levels in jejunal mucosa induced by TGEV, and maintained intestinal barrier integrity (P < 0.05). Meanwhile, 5 mg/d ATRA feeding increased the sucrase activity and the expressions of nutrient transporter related genes (GLUT2 and SLC7A1) in jejunal mucosa of TGEV-challenged piglets (P < 0.05). Furthermore, 5 mg/d ATRA feeding attenuated TGEV-induced intestinal inflammatory response by inhibiting the release of interleukin (IL)-1β, IL-8 and tumor necrosis factor-α (TNF-α), and promoting the secretion of IL-10 and secretory immunoglobulin A (sIgA) (P < 0.05). Feeding 5 mg/d ATRA also down-regulated the expressions of Toll-like receptors and RIG-I like receptors signaling pathway related genes (TLR3, TLR4, RIG-I, MyD88, TRIF and MAVS) and the phosphorylation level of nuclear factor-κB-p65 (NF-κB p65), and up-regulated the inhibitor kappa B alpha (IκBα) protein level in jejunal mucosa of TGEV-challenged piglets (P < 0.05).

Conclusions: ATRA alleviated TGEV-induced intestinal barrier damage by inhibiting inflammatory response, thus improving the growth performance and inhibiting diarrhea of piglets. The mechanism was associated with the inhibition of NF-κB signaling pathway mediated by TLR3, TLR4 and RIG-I.

全反式维甲酸可减轻传染性胃肠炎病毒引起的断奶仔猪肠道炎症和屏障功能障碍。
背景:传染性胃肠炎病毒(TGEV)是导致仔猪严重腹泻的主要病原体之一。TGEV 的发病机制与肠道炎症密切相关。全反式维甲酸(ATRA)是维生素 A 的主要活性代谢产物,具有免疫调节和抗炎特性。然而,ATRA 能否缓解 TGEV 引起的仔猪肠道炎症和屏障功能障碍尚不清楚。本研究旨在探讨 ATRA 对 TGEV 感染仔猪的生长性能、腹泻、肠道炎症和肠屏障完整性的影响:在一项为期 19 天的研究中,32 头断奶仔猪被随机分为 4 个处理:对照组(基础日粮)、TGEV 组(基础日粮 + TGEV 挑战)、TGEV + ATRA5 组(基础日粮 + 5 mg/d ATRA + TGEV 挑战)和 TGEV + ATRA15 组(基础日粮 + 15 mg/d ATRA + TGEV 挑战)。第 14 天,给仔猪口服 TGEV 或无菌培养基:结果:给仔猪饲喂 5 毫克和 15 毫克/天的 ATRA 可减轻 TGEV 引起的生长抑制和腹泻(P 结论):ATRA 通过抑制炎症反应减轻了 TGEV 引起的肠屏障损伤,从而改善了仔猪的生长性能并抑制了腹泻。其机制与抑制 TLR3、TLR4 和 RIG-I 介导的 NF-κB 信号通路有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.30
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822
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