The Implication of Growth Arrest-Specific 5 rs145204276 Polymorphism and Serum Expression of Sirtuin 1, Transforming Growth Factor-Beta, and microRNA-182 in Breast Cancer.

IF 1.9 4区医学 Q3 ONCOLOGY

Clinical Medicine Insights-Oncology Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI:10.1177/11795549241227415

Nabil A Hasona, Mahmoud Elsabahy, Olfat G Shaker, Othman Zaki, Ghada Ayeldeen

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引用次数: 0

Abstract

Background: Breast cancer (BC) patients have a higher chance of survival if it is diagnosed at an early stage, which is essential for efficient treatment of the condition. The results of an elevated risk of cancer, including BC, previously associated with the ins/del polymorphism rs145204276 in the promoter region of growth arrest-specific 5 (GAS5) are still up for debate. Thus, this study aimed to appraise the frequency of the GAS5 rs145204276 variant with BC risk and demonstrate the potential impact of the sirtuin 1 (SIRT-1), transforming growth factor-beta (TGF-β), and microRNA-182 (miR-182) expression and their diagnostic value in BC.

Methods: Blood samples of 155 patients with BC and fibroadenoma and 80 healthy controls were analyzed for GAS5 rs145204276 single nucleotide polymorphism (SNP), SIRT-1, TGF-β, and miRNA-182 expression levels.

Results: Ins/ins genotype and ins allele frequencies for GAS5 rs145204276 were considerably higher in BC patients compared with controls. Patients with BC had significantly greater serum levels of TGF-β, miR-182, and SIRT-1 expression.

Conclusions: The SIRT-1, TGF-β, and miR-182 genes provide novel, noninvasive diagnostic biomarkers for BC.

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生长停滞特异性 5 rs145204276 多态性与乳腺癌中 Sirtuin 1、转化生长因子-Beta 和 microRNA-182 的血清表达的影响

背景：乳腺癌（BC）患者如果能在早期阶段得到诊断，生存几率会更高，这对有效治疗乳腺癌至关重要。关于生长停滞特异性 5（GAS5）启动子区域中的 ins/del 多态性 rs145204276 与包括 BC 在内的癌症风险升高的关系，目前仍有争议。因此，本研究旨在评估GAS5 rs145204276变异与BC风险的频率，并证明sirtuin 1（SIRT-1）、转化生长因子-β（TGF-β）和microRNA-182（miR-182）表达的潜在影响及其在BC中的诊断价值：方法：分析155名BC和纤维腺瘤患者及80名健康对照者的血样，检测GAS5 rs145204276单核苷酸多态性（SNP）、SIRT-1、TGF-β和miRNA-182的表达水平：与对照组相比，GAS5 rs145204276的Ins/ins基因型和ins等位基因频率在BC患者中明显较高。BC患者血清中TGF-β、miR-182和SIRT-1的表达水平明显更高：结论：SIRT-1、TGF-β和miR-182基因为BC提供了新的、非侵入性的诊断生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Medicine Insights-Oncology Medicine-Oncology

CiteScore

2.40

自引率

4.50%

发文量

审稿时长

8 weeks

期刊介绍： Clinical Medicine Insights: Oncology is an international, peer-reviewed, open access journal that focuses on all aspects of cancer research and treatment, in addition to related genetic, pathophysiological and epidemiological topics. Of particular but not exclusive importance are molecular biology, clinical interventions, controlled trials, therapeutics, pharmacology and drug delivery, and techniques of cancer surgery. The journal welcomes unsolicited article proposals.