Fatty acid modification of cultured neuroblastoma cells by gamma linolenic acid relevant to its antitumor effect

Fumihiro Fujiwara, Shinjiro Todo, Shinsaku Imashuku
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引用次数: 18

Abstract

Two human neuroblastoma cell lines, NCG and GOTO, were used to study the cytotoxic effect of gamma linolenic acid (GLA). The cell growth inhibition of these culture cells by GLA was found to be associated with striking membrane fatty acid modification.When culture cells were exposed to 20 μg/ml and 60 μg/ml GLA for 48 hr, polyenoic acids in cell membrane phospholipids (PC, PE, PI, PS) and triglyceride significantly increased; 1.8–21.0 fold for NCG and 1.04–11.5 fold for GOTO, in association with decreased monoenoic acids. The most remarkable changes were; increase of C18:3, C20:3, C20:4 and decrease of C18:1. CoQ10 (50 μg/ml) and vitamin E (10 μM) shown to protect against the cytotoxic effect of GLA did not modify the incorporation of GLA into tumor cells.

These results indicate that the antitumor effect of GLA is probably due to cellular dysfunction caused by fatty acid modification after GLA incorporation.

γ -亚麻酸对培养神经母细胞瘤细胞的脂肪酸修饰及其抗肿瘤作用
用两种人神经母细胞瘤细胞系NCG和GOTO研究了γ -亚麻酸(GLA)的细胞毒性作用。发现GLA对这些培养细胞的细胞生长抑制与显著的膜脂肪酸修饰有关。20 μg/ml和60 μg/ml GLA作用细胞48小时后,细胞膜磷脂(PC、PE、PI、PS)和甘油三酯中多烯酸含量显著升高;NCG为1.8-21.0倍,GOTO为1.04-11.5倍,与单烯酸减少有关。最显著的变化是;C18:3、C20:3、C20:4增加,C18:1减少。CoQ10 (50 μg/ml)和维生素E (10 μM)对GLA的细胞毒作用有保护作用,但对GLA进入肿瘤细胞没有影响。这些结果表明,GLA的抗肿瘤作用可能与GLA掺入后脂肪酸修饰引起的细胞功能障碍有关。
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