A. A. Darbinyan, L. M. Parseghyan, A. V. Moghrovyan, M. A. Babajanyan, A. V. Voskanyan
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引用次数: 0
Abstract
Blunt-nosed viper venom in high doses causes severe pain, while in low doses an analgesic effect is observed. The opposite effects of different doses of venom are associated with the development of certain metabolic processes and the impact on receptors and channels of nociceptive afferent neurons. The response of the nociceptive system in the periphery and CNS depends on the dose-evoked neurochemical changes in neuronal activity. We studied the effect of various doses of MLO venom in order to understand the mechanisms of the transition of the pain effect of MLO venom into an analgesic one. Pain behavior was studied on outbred white mice at various doses of MLO venom, starting from LD50 for intraplantar and in serial dilutions for intraperitoneal (1.0 and 1/5, 1/10, 1/20 and 1/30 of LD50) administration during the formalin and the hot plate tests. At a dose of 1.0 LD50, experimental mice develop a strong sense of pain, which was examined in the hind paw biting/licking test. The maximum analgesic effect was expressed at 1/20 LD50. To study the degree of participation of the venom’s phospholipase A2 (PLA2) enzymatic activity on pain processes, a venom with the inhibited enzymatic activity of PLA2 was tested. It was obtained, that both in the development of pain and in the analgesic effect, the enzymatic activity of PLA2 plays a significant role. It is proposed that in case of high doses the antinociceptive action of venom is masked.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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