Mechanistic characterization of waterborne selenite uptake in the water flea, Daphnia magna, indicates water chemistry affects toxicity in coal mine-impacted waters.

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Conservation Physiology Pub Date : 2024-01-27 eCollection Date: 2024-01-01 DOI:10.1093/conphys/coad108
Chantelle E Klaczek, Greg G Goss, Chris N Glover
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引用次数: 0

Abstract

Concentrations of selenium that exceed regulatory guidelines have been associated with coal mining activities and have been linked to detrimental effects on aquatic ecosystems and the organisms therein. Although the major route of selenium uptake in macroinvertebrates is via the diet, the uptake of waterborne selenite (HSeO3-), the prominent form at circumneutral pH, can be an important contributor to selenium body burden and thus selenium toxicity. In the current study, radiolabelled selenite (Se75) was used to characterize the mechanism of selenite uptake in the water flea, Daphnia magna. The concentration dependence (1-32 μM) of selenite uptake was determined in 1-hour uptake assays in artificial waters that independently varied in bicarbonate, chloride, sulphate, phosphate and selenate concentrations. At concentrations representative of those found in highly contaminated waters, selenite uptake was phosphate-dependent and inhibited by foscarnet, a phosphate transport inhibitor. At higher concentrations, selenite uptake was dependent on waterborne bicarbonate concentration and inhibited by the bicarbonate transporter inhibitor DIDS (4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid). These findings suggest that concentrations of phosphate in coal mining-affected waters could alter selenite uptake in aquatic organisms and could ultimately affect the toxic impacts of selenium in such waters.

水蚤 Daphnia magna 吸收水载亚硒酸盐的机理特征表明,水化学影响煤矿影响水域的毒性。
硒的浓度超过监管准则与煤矿开采活动有关,并对水生生态系统和其中的生物产生有害影响。虽然大型无脊椎动物摄取硒的主要途径是通过饮食,但摄取水载亚硒酸盐(HSeO3-)(在环中性 pH 值下的主要形式)可能是造成硒的体内负荷并进而产生硒毒性的重要因素。本研究利用放射性标记亚硒酸盐(Se75)来描述水蚤 Daphnia magna 吸收亚硒酸盐的机制。在碳酸氢盐、氯化物、硫酸盐、磷酸盐和硒酸盐浓度各不相同的人工水体中进行的 1 小时吸收试验中,测定了亚硒酸盐吸收的浓度依赖性(1-32 μM)。在具有代表性的高污染水体中,亚硒酸盐的吸收依赖于磷酸盐,并受到磷酸盐转运抑制剂 foscarnet 的抑制。在较高浓度下,亚硒酸盐的吸收取决于水中碳酸氢盐的浓度,并受到碳酸氢盐转运抑制剂 DIDS(4,4'-二异硫氰基-2,2'-二苯乙烯二磺酸)的抑制。这些发现表明,受采煤影响的水域中磷酸盐的浓度可能会改变水生生物对亚硒酸盐的吸收,并最终影响这些水域中硒的毒性影响。
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来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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