Deciphering molecular basis of pesticide-induced recurrent pregnancy loss: insights from transcriptomics analysis.

IF 3.2 4区医学 Q1 Pharmacology, Toxicology and Pharmaceutics

Toxicology Mechanisms and Methods Pub Date : 2024-06-01 Epub Date: 2024-01-31 DOI:10.1080/15376516.2024.2307975

Muhammad Luthfi, R B Pandey, Yong-Chao Su, Pornthep Sompornpisut

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引用次数: 0

Abstract

Recent studies have revealed a notable connection between pesticide exposure and Recurrent Pregnancy Loss (RPL), yet the precise molecular underpinning of this toxicity remains elusive. Through the alignment of Differentially Expressed Genes (DEGs) of healthy and RPL patients with the target genes of 9 pesticide components, we identified a set of 12 genes responsible for RPL etiology. Interestingly, biological process showed that besides RPL, those 12 genes also associated with preeclampsia and cardiovascular disease. Enrichment analysis showed the engagement of these genes associated with essential roles in the molecular transport of small molecules, as well as the aldosterone-regulated sodium reabsorption, endocrine and other factor-regulated calcium reabsorption, mineral absorption, ion homeostasis, and ion transport by P-type ATPases. Notably, the crosstalk targets between pesticide components played crucial roles in influencing RPL results, suggesting a role in attenuating pesticide agents that contribute to RPL. It is important to note that non-significant concentration of the pesticide components observed in both control and RPL samples should not prematurely undermine the potential for pesticides to induce RPL in humans. This study emphasizes the complexity of pesticide induced RPL and highlights avenues for further research and precautionary measures.

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解密农药诱发复发性妊娠失败的分子基础：转录组学分析的启示。

最近的研究揭示了农药暴露与复发性妊娠失败（RPL）之间的显著联系，但这种毒性的确切分子基础仍然难以捉摸。通过将健康人和 RPL 患者的差异表达基因（DEGs）与 9 种农药成分的靶基因进行比对，我们发现了一组 12 个导致 RPL 病因的基因。有趣的是，生物学过程显示，除 RPL 外，这 12 个基因还与子痫前期和心血管疾病相关。富集分析表明，这些基因在小分子分子转运、醛固酮调控的钠重吸收、内分泌和其他因子调控的钙重吸收、矿物质吸收、离子稳态以及 P 型 ATP 酶的离子转运中发挥着重要作用。值得注意的是，农药成分之间的串扰靶标在影响 RPL 结果方面发挥了关键作用，这表明农药成分在减弱导致 RPL 的作用方面发挥了作用。值得注意的是，在对照组和 RPL 样品中观察到的农药成分浓度不显著，不应过早地削弱农药诱发人类 RPL 的可能性。这项研究强调了农药诱发 RPL 的复杂性，并突出了进一步研究和预防措施的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods 医学-毒理学

CiteScore

6.60

自引率

3.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.