Characterization of Prognostic Apoptosis-Related Gene Signature to Evaluate Glioma Immune Microenvironment and Experimental Verification.

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY
Hao Yu, Jiapeng Yu, Minjie Wang, Xiaobing Jiang
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引用次数: 0

Abstract

Purpose: Recently, apoptosis-related genes were shown to modulate cancer immunity. However, the role of apoptosis-related genes in the glioma immune microenvironment (GIME) remains unknown. This study aimed to explore the prognostic value of apoptosis-related genes in glioma. Methods: Doxorubicin was used to induce glioma cell apoptosis, and four differentially expressed apoptosis-related genes were identified: CREM, TNFSF12, PEA15, and PRKCD. Kaplan-Meier analyses, receiver operating characteristic curve analyses, and nomograms were established to determine the relationship between risk markers and the prognosis of patients with glioma. Results: Risk biomarkers were significantly associated with overall survival, immune cell infiltration, and immune checkpoints in patients with glioma. Somatic mutations and anti-PD-1/L1 immunotherapy were associated with worse prognosis in the high-risk group receiving anti-PD-1/L1 therapy. The expression of these four apoptosis-related genes was verified using quantitative polymerase chain reaction and immunohistochemistry, and the relationship between these four genes and apoptosis was examined using flow cytometry. Conclusions: This study suggests that apoptosis-related genes play a critical role in shaping the GIME. Assessing the apoptotic patterns of individual tumors will enhance our understanding of GIME infiltration features and lead to improved strategies for immunotherapy.

评估胶质瘤免疫微环境的预后性凋亡相关基因特征及实验验证
目的:最近,凋亡相关基因被证明可调节癌症免疫。然而,凋亡相关基因在胶质瘤免疫微环境(GIME)中的作用仍然未知。本研究旨在探讨胶质瘤中凋亡相关基因的预后价值。研究方法使用多柔比星诱导胶质瘤细胞凋亡,并鉴定了四个差异表达的凋亡相关基因:CREM、TNFSF12、PEA15和PRKCD。通过卡普兰-梅耶分析、接收器操作特征曲线分析和提名图来确定风险标志物与胶质瘤患者预后之间的关系。结果显示风险生物标记物与胶质瘤患者的总生存期、免疫细胞浸润和免疫检查点有明显相关性。在接受抗PD-1/L1治疗的高危人群中,体细胞突变和抗PD-1/L1免疫治疗与预后恶化有关。利用定量聚合酶链反应和免疫组化技术验证了这四个与细胞凋亡相关的基因的表达,并利用流式细胞术研究了这四个基因与细胞凋亡之间的关系。研究结论这项研究表明,与细胞凋亡相关的基因在形成 GIME 的过程中起着至关重要的作用。评估单个肿瘤的凋亡模式将加深我们对GIME浸润特征的了解,从而改进免疫疗法策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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