Prospects for the creation of an oral vaccine against ornithobacteriosis of birders

Abstract

   The work was carried out at the pharmacogenomics laboratory of the Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences and in the molecular biology sector of the Siberian Federal Scientific Center for Agrobiotechnologies of the Russian Academy of Sciences. Ornithobacterium rhinotracheale DNA was provided by poultry farms of the Republic of Mordovia of the Russian Federation. In affected birds, inflammatory changes were observed in the larynx, upper part of the trachea and infraorbital sinuses, and fibrin clots were observed in the respiratory sacs. Pathological material was subcultured in brain heart broth in the presence of antibiotics. Analysis of subcellular localisation of the OR77 protein was carried out using the SignalP-6.0 method. The immune epitope database (IEDB) was used to search for B-dependent epitopes of the OR77 gene. Primers for cloning were designed, and cloning regions of the OR77 gene containing B-dependent epitopes were simulated by ligating BamHI and HindIII into the polylinker of the shuttle vector pBE-S. Experiments were carried out to clone the active epitope sequence of the O. rhinotracheale OR77 protein in Bacillus subtilis and use it orally to identify immune activity and other possible adverse reactions. Nucleotide sequence analysis was performed using the UniproUGENEv program. The amplification procedure was carried out. The prospect of the possible synthesis of a new, inexpensive, and effective vaccine against the pathogen O. rhinotracheale is described.