Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020

Pub Date : 2024-01-18 DOI:10.12681/jhvms.31543

BS Tokgoz, EA Tokgoz, MA Sözmen, O. Avci, AE Ütük

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Abstract

The aim of this study is to investigate the presence of BEF disease in the blood, sera and tissue samples sent to the Viral Diagnosis Laboratory of Adana Veterinary Control Institute with suspected BEF in 2020 from different provinces of Turkey as well as to perform characterization and phylogenetic analysis of cases tested positive with molecular methods. For this purpose, 79 defibrinated blood and 2 tissue samples as well as 168 sera samples obtained from cattle varying in breed, age, and sex, were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed and the phylogenetic analysis of the samples tested positive with RT-PCR was performed. As a result of the examinations conducted, 64.20% (52/81) of the defibrinated blood and tissue samples, and 69.04% (116/168) of the sera samples were diagnosed as positive. Through VERO cell culture, the virus was isolated from the tissue samples of 2 animals in Adana and Sanliurfa provinces, which were positive. The phylogenetic analysis revealed that the virus circulated in Turkey during the 2020 pandemic belongs to the Middle East lineage. We concluded that monitoring the disease and identifying the lineages of the virus that have caused pandemic are significant in the selection of the proper vaccine and the disease control.

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2020 年疫情爆发时土耳其南部和东南部地区牛短暂热病毒的流行、分离和分子特征描述

本研究的目的是调查 2020 年土耳其不同省份送往阿达纳兽医控制研究所病毒诊断实验室的疑似 BEF 牛的血液、血清和组织样本中是否存在 BEF 病，并对分子方法检测呈阳性的病例进行特征描述和系统发育分析。为此，我们采用实时 RT-PCR 和阻断酶联免疫吸附法分别检测了从不同品种、年龄和性别的牛身上采集的 79 份脱纤维血液样本和 2 份组织样本以及 168 份血清样本。设计了两个新的退化引物（AVKEF_AATGTTCCNGTGAATTGTGGAG 和 AVKER_TGCATAATCYCTTCCTGGTCT）扩增蛋白 G 的 956 bp，并对 RT-PCR 检测呈阳性的样本进行了系统发育分析。检查结果显示，64.20%（52/81）的脱纤血液和组织样本以及 69.04%（116/168）的血清样本被诊断为阳性。通过 VERO 细胞培养，从阿达纳省和桑尼乌尔法省的 2 只动物的组织样本中分离出了病毒，结果呈阳性。系统进化分析表明，2020 年大流行期间在土耳其流行的病毒属于中东系。我们的结论是，监测疾病和确定导致大流行的病毒系对于选择适当的疫苗和控制疾病具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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