Presence of genes encoding aminoglycoside-modifying enzyme (AME) and virulence factors in high-level aminoglycoside-resistant (HLAR) Enterococcus strains isolated from retail chicken meat in Turkey

Pub Date : 2024-01-18 DOI:10.12681/jhvms.30850

M. Yalçın, Özden Tuncer, Akpınar Kankaya, Y. Tuncer, B. Ö. Tuncer, D. Kankaya

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Abstract

In this study, the presence of aminoglycoside-modifying enzyme (AME) and virulence factor genes were investigated in previously isolated 32 high-level aminoglycoside-resistant (HLAR) Enterococcus strains isolated from retail chicken meat in Turkey. At least one AME-encoding gene was detected in HLAR enterococci by polymerase chain reaction (PCR). The ant(6ʹ)-Ia was identified as the most prevalent (87.5%, 28/32) AME gene. The aph(3ʹ)-IIIa (78.13%, 25/32), ant(4ʹ)-Ia (68.75%, 22/32), aph(2ʹʹ)-Ib (62.5%, 20/32), aac(6ʹ)-Ie-aph(2ʹʹ)-Ia (21.88%, 7/32) and aph(2ʹʹ)-Ic (9.38%, 3/32) are the other detected AME-encoding genes in strains. The aph(2ʹʹ)-Id was found in none of the HLAR strains. The aph(2ʹʹ)-Ib and ant(6ʹ)-Ia were identified as the most frequently AME-encoding genes in high-level gentamicin-resistant (HLGR) and high-level streptomycin-resistant (HLSR) strains, respectively. Among the 32 HLAR strains, only E. faecalis MSE61.1 and E. avium MSE63.1 were found capable of hydrolyzing gelatine. All HLAR strains showed α-hemolytic activity except E. durans MG13.4 and E. casseliflavus MGM111.1, which were exhibited β- and γ-hemolytic activity, respectively. It was determined that all HLAR strains, except E. durans MGE13.1 and MGE63.1, contain at least one virulence factor gene. The efaAfm (87.5%, 28/32), acm (65.63%, 21/32) and gelE (37.5%, 12/32) were found to be the most prevalent virulence factor genes. HLAR enterococci strains that have the virulence factor genes may pose a risk to consumer health.

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从土耳其零售鸡肉中分离出的高水平氨基糖苷类药物耐药 (HLAR) 肠球菌菌株中存在编码氨基糖苷类药物修饰酶 (AME) 的基因和毒力因子

本研究调查了之前从土耳其零售鸡肉中分离出的 32 株高水平氨基糖苷类耐药肠球菌（HLAR）中是否存在氨基糖苷修饰酶（AME）和毒力因子基因。通过聚合酶链反应（PCR），在 HLAR 肠球菌中至少检测到一个 AME 编码基因。经鉴定，ant(6ʹ)-Ia 是最常见的 AME 基因（87.5%，28/32）。aph(3ʹ)-IIIa（78.13%，25/32）、ant(4ʹ)-Ia（68.75%，22/32）、aph(2ʹʹ)-Ib（62.5%，20/32）、ac(6ʹ)-Ie-aph(2ʹʹ)-Ia（21.88%, 7/32) 和 aph(2ʹʹ)-Ic (9.38%, 3/32) 是在菌株中检测到的其他 AME 编码基因。HLAR菌株中没有发现ph(2ʹʹ)-Id基因。高水平庆大霉素耐药菌株（HLGR）和高水平链霉素耐药菌株（HLSR）中最常见的 AME 编码基因分别是 aph(2ʹʹ)-Ib 和 ant(6ʹ)-Ia 。在 32 株 HLAR 菌株中，只有粪肠球菌 MSE61.1 和阿维菌 MSE63.1 能够水解明胶。除 E. durans MG13.4 和 E. casseliflavus MGM111.1 分别具有 β 和 γ 溶血活性外，所有 HLAR 菌株都具有 α 溶血活性。据测定，除杜兰氏大肠杆菌 MGE13.1 和 MGE63.1 外，所有 HLAR 菌株都至少含有一个毒力因子基因。发现 efaAfm（87.5%，28/32）、acm（65.63%，21/32）和 gelE（37.5%，12/32）是最普遍的毒力因子基因。含有致病因子基因的 HLAR 肠球菌菌株可能会对消费者的健康构成风险。

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