RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs

Pharmacognosy Magazine Pub Date : 2024-01-18 DOI:10.1177/09731296231204762

Liying Zhang, Ting Zhou, Yi-ming Zhang, Lei Wang, Yangyang Li, Gucheng Zhou, Heng Zhou, Fan Niu, Zhiwei Liu, Zhiming Miao, Yongqi Liu

{"title":"RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs","authors":"Liying Zhang, Ting Zhou, Yi-ming Zhang, Lei Wang, Yangyang Li, Gucheng Zhou, Heng Zhou, Fan Niu, Zhiwei Liu, Zhiming Miao, Yongqi Liu","doi":"10.1177/09731296231204762","DOIUrl":null,"url":null,"abstract":"Ionizing radiation can induce bystander effects (RIBE), and astragalus polysaccharides (APS) have a protective effect against RIBE. It has been proved that RIBE occurred and APS played the inhibitory effects on the RIBE when radiation was carried out only one time. However, whether RIBE happened by multiple irradiations and the effects of APS on the RIBE are unclear. This study aimed to investigate whether APS suppress RIBE damage induced by multiple irradiations. A549 cells were irradiated with 2 Gy X-rays to obtain a conditioned medium. Bone mesenchymal stem cells (BMSCs) was incubated with the conditioned medium 5 times every 3 days or APS. Cell proliferation was detected by CCK-8 and colony formation assay, and genomic instability and DNA damages were detected by the micronucleus and immunofluorescence assay. The protein associated with methylation and cancer-associated proteins in BMSCs were detected by western blot. After five stimulations with a conditioned medium, the proliferative capacity of BMSCs decreased; APS pre-intervention promoted the proliferation of BMSCs. And conditional medium intervention increased the micronucleus rate and a number of 53BP1 foci; APS pre-intervention reduced the micronucleus rate and number of 53BP1 foci. In addition, the conditional medium intervention reduced the expression of methylation-related proteins and increased the expression of cancer-related proteins, while APS pre-intervention reversed this trend. Irradiated A549 conditioned medium can induce RIBE of BMSCs, which might be related to methylation and cancer-associated proteins and APS may block RIBE by multiple irradiations in BMSCs.","PeriodicalId":508089,"journal":{"name":"Pharmacognosy Magazine","volume":"115 25","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacognosy Magazine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/09731296231204762","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Ionizing radiation can induce bystander effects (RIBE), and astragalus polysaccharides (APS) have a protective effect against RIBE. It has been proved that RIBE occurred and APS played the inhibitory effects on the RIBE when radiation was carried out only one time. However, whether RIBE happened by multiple irradiations and the effects of APS on the RIBE are unclear. This study aimed to investigate whether APS suppress RIBE damage induced by multiple irradiations. A549 cells were irradiated with 2 Gy X-rays to obtain a conditioned medium. Bone mesenchymal stem cells (BMSCs) was incubated with the conditioned medium 5 times every 3 days or APS. Cell proliferation was detected by CCK-8 and colony formation assay, and genomic instability and DNA damages were detected by the micronucleus and immunofluorescence assay. The protein associated with methylation and cancer-associated proteins in BMSCs were detected by western blot. After five stimulations with a conditioned medium, the proliferative capacity of BMSCs decreased; APS pre-intervention promoted the proliferation of BMSCs. And conditional medium intervention increased the micronucleus rate and a number of 53BP1 foci; APS pre-intervention reduced the micronucleus rate and number of 53BP1 foci. In addition, the conditional medium intervention reduced the expression of methylation-related proteins and increased the expression of cancer-related proteins, while APS pre-intervention reversed this trend. Irradiated A549 conditioned medium can induce RIBE of BMSCs, which might be related to methylation and cancer-associated proteins and APS may block RIBE by multiple irradiations in BMSCs.

查看原文本刊更多论文

多重辐照的 RIBE 以及黄芪多糖对 BMSCs 甲基化和癌症相关蛋白的保护作用

电离辐射可诱发旁观者效应（RIBE），而黄芪多糖（APS）对RIBE具有保护作用。研究证明，只照射一次就会发生 RIBE，而 APS 对 RIBE 有抑制作用。然而，多次照射是否会发生 RIBE 以及 APS 对 RIBE 的影响尚不清楚。本研究旨在探讨APS是否能抑制多次辐照引起的RIBE损伤。用 2 Gy X 射线照射 A549 细胞以获得条件培养基。将骨间充质干细胞（BMSCs）与条件培养基或 APS 一起培养，每 3 天 5 次。细胞增殖通过 CCK-8 和集落形成试验检测，基因组不稳定性和 DNA 损伤通过微核和免疫荧光试验检测。用 Western 印迹法检测 BMSCs 中与甲基化相关的蛋白和癌症相关蛋白。经过五次条件培养基刺激后，BMSCs 的增殖能力下降；APS 预干预可促进 BMSCs 的增殖。而条件培养基干预增加了微核率和 53BP1 病灶的数量；APS 预干预降低了微核率和 53BP1 病灶的数量。此外，条件培养基干预降低了甲基化相关蛋白的表达，增加了癌症相关蛋白的表达，而 APS 预干预则逆转了这一趋势。辐照过的A549条件培养基可诱导BMSCs的RIBE，这可能与甲基化和癌症相关蛋白有关，而APS可通过多次辐照阻断BMSCs的RIBE。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pharmacognosy Magazine

自引率

0.00%

发文量