Molecular characterization of extended-spectrum beta-lactamases and carbapenemases producing Enterobacteriaceae isolated from North Eastern region of India

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL

Journal of Laboratory Physicians Pub Date : 2024-01-23 DOI:10.25259/jlp-2023-5-17-(1795)

Thounaojam Salvia, Laishram Shantikumar Singh, Rachana Khati, Kalaiarasan Ellappan, K. Dolma, Om Prakash Dhakal

{"title":"Molecular characterization of extended-spectrum beta-lactamases and carbapenemases producing Enterobacteriaceae isolated from North Eastern region of India","authors":"Thounaojam Salvia, Laishram Shantikumar Singh, Rachana Khati, Kalaiarasan Ellappan, K. Dolma, Om Prakash Dhakal","doi":"10.25259/jlp-2023-5-17-(1795)","DOIUrl":null,"url":null,"abstract":"\n\nThis study is aimed to investigate the prevalence of genes encoding extended-spectrum β-lactamases (ESBLs) and carbapenemases production among Enterobacteriaceae isolated from North East India.\n\n\n\nA total of 210 non-duplicate multi-drug resistant Enterobacteriaceae (MDRE) strains were included in this investigation. The isolates were resistant to third-generation cephalosporins, aminoglycosides, and fluoroquinolones. First, the strains were subjected to phenotypic assays to determine ESBLs and carbapenemases production; then, multiplex polymerase chain reaction (mPCR) assays were done to detect ESBLs and carbapenemases genes. In addition, efflux pump activity was determined by phenylalanine-arginine b-naphthylamide assay.\n\n\n\nThe frequency of ESBLs and carbapenemase genes among MDRE strains was shown as percentages. The data analysis was done using Microsoft Excel computer software.\n\n\n\nAmong 210 MDRE clinical isolates, ESBLs production was observed in 72.86% (153) isolates. During mPCR assay, gene encoding ESBLs were detected in 55.24% (116) MDRE strains beta-lactamase Temoniera (blaTEM) (26.67%, 56), beta-lactamase Cefotaxime-Munich (blaCTX-M) (19.52%, 41), and beta-lactamase sulfhydryl reagent variable (blaSHV) (9.05%, 19)]. In addition, 55 (26.2%) and 53 (25.26%) strains were found to be meropenem and imipenem resistant, respectively. Carbapenemase nordmann-poirel (Carba-NP) test for carbapenemases activity was found to be positive in 18.58% (39) MDRE strains. The genes encoding carbapenemases production was observed in 18.58% (39) MDRE [beta-lactamase New Delhi metallo-β-lactamases-1(blaNDM-1) (8.10%, 17), beta-lactamase oxacillinase-48 (blaOXA-48) (2.86%, 6), beta-lactamase Verona imipenemase (blaVIM) (1.43%, 3), and blaOXA-48 and blaVIM (6.19%, 13)]. Efflux pump activity was observed in 5 (2.3%) of Carbapenem-resistant Enterobacteriaceae isolates.\n\n\n\nFor the first time in this region, we have detected the presence of blaOXA-48 and blaVIM in a single MDRE isolate as high as 6.1%. Therefore, clinicians need to detect the ESBLs and carbapenemases producing Enterobacteriaceae on priority in hospital settings for therapeutic options as well as stringent infection control strategies to be adopted as precautions.\n","PeriodicalId":16149,"journal":{"name":"Journal of Laboratory Physicians","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Laboratory Physicians","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25259/jlp-2023-5-17-(1795)","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

This study is aimed to investigate the prevalence of genes encoding extended-spectrum β-lactamases (ESBLs) and carbapenemases production among Enterobacteriaceae isolated from North East India. A total of 210 non-duplicate multi-drug resistant Enterobacteriaceae (MDRE) strains were included in this investigation. The isolates were resistant to third-generation cephalosporins, aminoglycosides, and fluoroquinolones. First, the strains were subjected to phenotypic assays to determine ESBLs and carbapenemases production; then, multiplex polymerase chain reaction (mPCR) assays were done to detect ESBLs and carbapenemases genes. In addition, efflux pump activity was determined by phenylalanine-arginine b-naphthylamide assay. The frequency of ESBLs and carbapenemase genes among MDRE strains was shown as percentages. The data analysis was done using Microsoft Excel computer software. Among 210 MDRE clinical isolates, ESBLs production was observed in 72.86% (153) isolates. During mPCR assay, gene encoding ESBLs were detected in 55.24% (116) MDRE strains beta-lactamase Temoniera (blaTEM) (26.67%, 56), beta-lactamase Cefotaxime-Munich (blaCTX-M) (19.52%, 41), and beta-lactamase sulfhydryl reagent variable (blaSHV) (9.05%, 19)]. In addition, 55 (26.2%) and 53 (25.26%) strains were found to be meropenem and imipenem resistant, respectively. Carbapenemase nordmann-poirel (Carba-NP) test for carbapenemases activity was found to be positive in 18.58% (39) MDRE strains. The genes encoding carbapenemases production was observed in 18.58% (39) MDRE [beta-lactamase New Delhi metallo-β-lactamases-1(blaNDM-1) (8.10%, 17), beta-lactamase oxacillinase-48 (blaOXA-48) (2.86%, 6), beta-lactamase Verona imipenemase (blaVIM) (1.43%, 3), and blaOXA-48 and blaVIM (6.19%, 13)]. Efflux pump activity was observed in 5 (2.3%) of Carbapenem-resistant Enterobacteriaceae isolates. For the first time in this region, we have detected the presence of blaOXA-48 and blaVIM in a single MDRE isolate as high as 6.1%. Therefore, clinicians need to detect the ESBLs and carbapenemases producing Enterobacteriaceae on priority in hospital settings for therapeutic options as well as stringent infection control strategies to be adopted as precautions.

查看原文本刊更多论文

从印度东北部地区分离的产广谱β-内酰胺酶和碳青霉烯酶的肠杆菌科细菌的分子鉴定

本研究旨在调查从印度东北部分离出的肠杆菌科细菌中编码广谱β-内酰胺酶（ESBLs）和碳青霉烯酶的基因的流行情况。这些分离菌株对第三代头孢菌素、氨基糖苷类和氟喹诺酮类药物具有耐药性。首先，对菌株进行表型检测，以确定 ESBLs 和碳青霉烯酶的产生情况；然后，进行多重聚合酶链反应（mPCR）检测，以检测 ESBLs 和碳青霉烯酶基因。MDRE菌株中ESBLs和碳青霉烯酶基因的频率以百分比表示。在 210 株 MDRE 临床分离株中，72.86%（153 株）的分离株产生了 ESBLs。在 mPCR 检测中，55.24%（116 株）MDRE 菌株检测到了编码 ESBLs 的基因：β-内酰胺酶 Temoniera（blaTEM）（26.67%，56 株）、β-内酰胺酶 Cefotaxime-Munich（blaCTX-M）（19.52%，41 株）和β-内酰胺酶巯基试剂变量（blaSHV）（9.05%，19 株）]。此外，还发现分别有 55 株（26.2%）和 53 株（25.26%）对美罗培南和亚胺培南耐药。在 18.58%（39 株）的 MDRE 菌株中，碳青霉烯酶诺德曼-泊雷尔（Carba-NP）检测呈阳性。在 18.58%（39 株）MDRE 菌株中观察到编码碳青霉烯酶的基因[β-内酰胺酶新德里金属-β-内酰胺酶-1（blaNDM-1）（8.10%，17），β-内酰胺酶氧青霉素酶-48（blaOXA-48）（2.86%，6），β-内酰胺酶维罗纳亚胺培南酶（blaVIM）（1.43%，3），以及 blaOXA-48 和 blaVIM（6.19%，13）]。在 5 个（2.3%）耐碳青霉烯类肠杆菌科细菌分离株中观察到了外排泵活性。在该地区，我们首次在单个 MDRE 分离株中检测到 blaOXA-48 和 blaVIM 的存在，比例高达 6.1%。因此，临床医生需要在医院环境中优先检测出产ESBLs和碳青霉烯酶的肠杆菌科细菌，以便选择治疗方案，并采取严格的感染控制策略作为预防措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-

自引率

0.00%

发文量

审稿时长

31 weeks