Green Synthesis of Gold and Copper Nanoparticles by Lannea discolor: Characterization and Antibacterial Activity

IF 3.1 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Inorganics Pub Date : 2024-01-24 DOI:10.3390/inorganics12020036

Unarine Rambau, Nndivhaleni Anox Masevhe, A. Samie

{"title":"Green Synthesis of Gold and Copper Nanoparticles by Lannea discolor: Characterization and Antibacterial Activity","authors":"Unarine Rambau, Nndivhaleni Anox Masevhe, A. Samie","doi":"10.3390/inorganics12020036","DOIUrl":null,"url":null,"abstract":"Green synthesis using plant extracts has emerged as an eco-friendly, clean, and viable alternative to chemical and physical approaches. Herein, the leaf, stem, and root extracts of Lannea discolor were utilized as a reducing and stabilizing agent in synthesizing gold (AuNPs) and copper (CuNPs) nanoparticles. The formation of AuNPs and CuNPs, confirmed by their color change, was characterized by UV-Vis spectroscopy (UV-Vis), scanning electron microscopy analysis, and energy-dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), coupled with minimum inhibitory concentration (MIC) antibacterial assays. Gold nanoflowers (AuNFs), NPs, and CuNPs peaked at wavelengths of 316, 544, and 564 nm, respectively. TEM showed unexpected nanoflowers (30–97 nm) in the leaf extracts and spherical NPs (10–33 nm; 9.3–37.5) from stem and root extracts, while spherical CuNPs (20–104 nm) were observed from all the extracts. EDX confirmed the presence of metal salts, and FTIR revealed stable capping agents. AuNPs and NFs from L. discolor extracts showed appreciable antibacterial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), and Bacillus subtilis (ATCC 6633) when compared to the plant extracts. At the same time, none was observed from the CuNPs. These AuNPs and CuNPs are particularly appealing in various biomedical and conductivity manufacturing applications due to their shapes and sizes and economical and environmentally friendly production. To our knowledge, this is the first study of the synthesis of gold and copper nanoparticles from L. discolor.","PeriodicalId":13572,"journal":{"name":"Inorganics","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Inorganics","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/inorganics12020036","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}

引用次数: 0

Abstract

Green synthesis using plant extracts has emerged as an eco-friendly, clean, and viable alternative to chemical and physical approaches. Herein, the leaf, stem, and root extracts of Lannea discolor were utilized as a reducing and stabilizing agent in synthesizing gold (AuNPs) and copper (CuNPs) nanoparticles. The formation of AuNPs and CuNPs, confirmed by their color change, was characterized by UV-Vis spectroscopy (UV-Vis), scanning electron microscopy analysis, and energy-dispersive X-ray (SEM-EDX), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), coupled with minimum inhibitory concentration (MIC) antibacterial assays. Gold nanoflowers (AuNFs), NPs, and CuNPs peaked at wavelengths of 316, 544, and 564 nm, respectively. TEM showed unexpected nanoflowers (30–97 nm) in the leaf extracts and spherical NPs (10–33 nm; 9.3–37.5) from stem and root extracts, while spherical CuNPs (20–104 nm) were observed from all the extracts. EDX confirmed the presence of metal salts, and FTIR revealed stable capping agents. AuNPs and NFs from L. discolor extracts showed appreciable antibacterial activity against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), and Bacillus subtilis (ATCC 6633) when compared to the plant extracts. At the same time, none was observed from the CuNPs. These AuNPs and CuNPs are particularly appealing in various biomedical and conductivity manufacturing applications due to their shapes and sizes and economical and environmentally friendly production. To our knowledge, this is the first study of the synthesis of gold and copper nanoparticles from L. discolor.

查看原文本刊更多论文

Lannea discolor 绿色合成金与铜纳米粒子：表征与抗菌活性

利用植物提取物进行绿色合成已成为一种环保、清洁、可行的化学和物理方法替代品。本文利用变色兰的叶片、茎和根提取物作为还原剂和稳定剂，合成金（AuNPs）和铜（CuNPs）纳米粒子。通过紫外-可见光谱（UV-Vis）、扫描电子显微镜分析、能量色散 X 射线（SEM-EDX）、透射电子显微镜（TEM）和傅立叶变换红外光谱（FTIR）以及最低抑菌浓度（MIC）抗菌检测，证实了 AuNPs 和 CuNPs 的形成。纳米金花（AuNFs）、NPs 和 CuNPs 的波长峰值分别为 316、544 和 564 纳米。TEM 显示，叶提取物中出现了意想不到的纳米花（30-97 nm），茎和根提取物中出现了球形 NPs（10-33 nm; 9.3-37.5），而所有提取物中都观察到了球形 CuNPs（20-104 nm）。EDX 证实了金属盐的存在，FTIR 揭示了稳定的封端剂。与植物提取物相比，从 L. discolor 提取物中提取的 AuNPs 和 NFs 对金黄色葡萄球菌（ATCC 25923）、大肠杆菌（ATCC 25922）、绿脓杆菌（ATCC 27853）、肺炎克雷伯氏菌（ATCC 700603）和枯草杆菌（ATCC 6633）具有明显的抗菌活性。与此同时，CuNPs 则没有任何作用。这些 AuNPs 和 CuNPs 因其形状和大小以及生产的经济性和环保性，在各种生物医学和导电性制造应用中特别具有吸引力。据我们所知，这是首次从 L. discolor 中合成金和铜纳米粒子的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Inorganics Chemistry-Inorganic Chemistry

CiteScore

2.80

自引率

10.30%

发文量

193

审稿时长

6 weeks

期刊介绍： Inorganics is an open access journal that covers all aspects of inorganic chemistry research. Topics include but are not limited to: synthesis and characterization of inorganic compounds, complexes and materials structure and bonding in inorganic molecular and solid state compounds spectroscopic, magnetic, physical and chemical properties of inorganic compounds chemical reactivity, physical properties and applications of inorganic compounds and materials mechanisms of inorganic reactions organometallic compounds inorganic cluster chemistry heterogenous and homogeneous catalytic reactions promoted by inorganic compounds thermodynamics and kinetics of significant new and known inorganic compounds supramolecular systems and coordination polymers bio-inorganic chemistry and applications of inorganic compounds in biological systems and medicine environmental and sustainable energy applications of inorganic compounds and materials MD