Bacterial Isolates from Diabetic Foot Ulcer with Antibiotic Sensitivity Pattern

Community Based Medical Journal Pub Date : 2024-01-24 DOI:10.3329/cbmj.v13i1.71087

Suttam Kumar Biswas, Shilpi Rani Roy, Arup Roton Paul

{"title":"Bacterial Isolates from Diabetic Foot Ulcer with Antibiotic Sensitivity Pattern","authors":"Suttam Kumar Biswas, Shilpi Rani Roy, Arup Roton Paul","doi":"10.3329/cbmj.v13i1.71087","DOIUrl":null,"url":null,"abstract":"A cross-sectional, descriptive study was carried out in the Department of Surgery, Mymensingh Medical College Hospital, Mymensingh, Bangladesh, between October 2014 and September 2015, to determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcer. A total of 130 patients of diabetic foot ulcer were included in the study according to inclusion and exclusion criteria. Samples of pus were collected from those patients and sent for culture and sensitivity tests. Out of 130 cases, the highest number of patients 55(42.4%) were in 50-59 years age group. The mean age was 60.1±9.8 years. Most of the patients (70%) were male. 90(69.2%) samples yielded growth, while 40(30.8%) did not show any bacterial growth. Out of those 90 samples with growths, 112 bacteria were isolated. Of them, 59(52.7%) organisms were gram-positive, while 28(25%) were gram-negative and 25(22.3%) organisms were both gram-positive and gram-negative. Out of 59 gram-positive isolates, 35(59.3%) were S. aureus, 18(30.5%) were Enterococci, and 6(10.2%) were Streptococci. In 28 gram-negative isolates, 15(53.7%) were E. coli, 6(21.4%) were Pseudomonas, 4(14.2%) were klebsiella, and 3(10.7%) were proteus. Regarding antibiotic sensitivity, all gram-positive bacteria (100%) were sensitive to vancomycin. S. aureus was 80% sensitive to ceftriaxone, 71.4% to flucloxacillin, 65.7% to clavulanic acid, 54.2% to ampicillin, 48.5% to amoxicillin, and 28.5% to cotrimoxazole. Enterococci spp. was 61.1% sensitive to ceftriaxone, 33.3% to clavulanic acid, (27.7%) to ampicillin, and 16.6% to both amoxicillin and cotrimoxazole. Streptococcus spp. showed 83.3% sensitivity to ceftriaxone, 66.6% to flucloxacillin, 33.3% to clavulanic acid, and 16.6% to cotrimoxazole. Among gram-negative bacteria, E. coli was found to be sensitive to imipenem (100%), ceftriaxone (86%), amikacin (100%), cefuroxime (73.3%), ampicillin (33.3%), ciprofloxacin (60%), and gentamycin (60%). Pseudomonas was found to be sensitive to imipenem (50%), ceftriaxone (66.6%), amikacin (83.3%), and cefuroxime (66.6%). Klebsiella was found to be sensitive to imipenem (100%), ceftriaxone (75%), amikacin (75%), cefuroxime (50%), and ciprofloxacin (25%). Proteus was found to be sensitive to imipenem (100%), ceftriaxone (66.6%), amikacin (66.6%), cefuroxime (33.3%), ciprofloxacin (66.6%), and gentamycin (66.6%). \nCBMJ 2024 January: vol. 13 no. 01 P: 89-94","PeriodicalId":10576,"journal":{"name":"Community Based Medical Journal","volume":"67 20","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Community Based Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3329/cbmj.v13i1.71087","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

A cross-sectional, descriptive study was carried out in the Department of Surgery, Mymensingh Medical College Hospital, Mymensingh, Bangladesh, between October 2014 and September 2015, to determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcer. A total of 130 patients of diabetic foot ulcer were included in the study according to inclusion and exclusion criteria. Samples of pus were collected from those patients and sent for culture and sensitivity tests. Out of 130 cases, the highest number of patients 55(42.4%) were in 50-59 years age group. The mean age was 60.1±9.8 years. Most of the patients (70%) were male. 90(69.2%) samples yielded growth, while 40(30.8%) did not show any bacterial growth. Out of those 90 samples with growths, 112 bacteria were isolated. Of them, 59(52.7%) organisms were gram-positive, while 28(25%) were gram-negative and 25(22.3%) organisms were both gram-positive and gram-negative. Out of 59 gram-positive isolates, 35(59.3%) were S. aureus, 18(30.5%) were Enterococci, and 6(10.2%) were Streptococci. In 28 gram-negative isolates, 15(53.7%) were E. coli, 6(21.4%) were Pseudomonas, 4(14.2%) were klebsiella, and 3(10.7%) were proteus. Regarding antibiotic sensitivity, all gram-positive bacteria (100%) were sensitive to vancomycin. S. aureus was 80% sensitive to ceftriaxone, 71.4% to flucloxacillin, 65.7% to clavulanic acid, 54.2% to ampicillin, 48.5% to amoxicillin, and 28.5% to cotrimoxazole. Enterococci spp. was 61.1% sensitive to ceftriaxone, 33.3% to clavulanic acid, (27.7%) to ampicillin, and 16.6% to both amoxicillin and cotrimoxazole. Streptococcus spp. showed 83.3% sensitivity to ceftriaxone, 66.6% to flucloxacillin, 33.3% to clavulanic acid, and 16.6% to cotrimoxazole. Among gram-negative bacteria, E. coli was found to be sensitive to imipenem (100%), ceftriaxone (86%), amikacin (100%), cefuroxime (73.3%), ampicillin (33.3%), ciprofloxacin (60%), and gentamycin (60%). Pseudomonas was found to be sensitive to imipenem (50%), ceftriaxone (66.6%), amikacin (83.3%), and cefuroxime (66.6%). Klebsiella was found to be sensitive to imipenem (100%), ceftriaxone (75%), amikacin (75%), cefuroxime (50%), and ciprofloxacin (25%). Proteus was found to be sensitive to imipenem (100%), ceftriaxone (66.6%), amikacin (66.6%), cefuroxime (33.3%), ciprofloxacin (66.6%), and gentamycin (66.6%). CBMJ 2024 January: vol. 13 no. 01 P: 89-94

查看原文本刊更多论文

从糖尿病足溃疡中分离出的细菌与抗生素敏感性模式

2014 年 10 月至 2015 年 9 月期间，孟加拉国迈门辛医学院附属医院外科开展了一项横断面描述性研究，以确定从糖尿病足溃疡中分离出的微生物概况和抗生素敏感性模式。根据纳入和排除标准，研究共纳入了 130 名糖尿病足溃疡患者。研究人员采集了这些患者的脓液样本，并送去进行培养和药敏试验。在 130 例患者中，55 例（42.4%）患者的年龄在 50-59 岁之间，占比最高。平均年龄为 60.1±9.8 岁。大多数患者（70%）为男性。90份样本（69.2%）显示有细菌生长，40份样本（30.8%）未显示任何细菌生长。在这 90 个有细菌生长的样本中，共分离出 112 种细菌。其中 59 个（52.7%）为革兰氏阳性菌，28 个（25%）为革兰氏阴性菌，25 个（22.3%）同时为革兰氏阳性菌和阴性菌。在 59 个革兰氏阳性分离物中，35 个（59.3%）为金黄色葡萄球菌，18 个（30.5%）为肠球菌，6 个（10.2%）为链球菌。在 28 株革兰氏阴性菌中，15 株（53.7%）为大肠杆菌，6 株（21.4%）为假单胞菌，4 株（14.2%）为克雷伯氏菌，3 株（10.7%）为变形杆菌。在抗生素敏感性方面，所有革兰氏阳性细菌（100%）都对万古霉素敏感。金黄色葡萄球菌对头孢曲松的敏感率为 80%，对氟氯西林的敏感率为 71.4%，对克拉维酸的敏感率为 65.7%，对氨苄西林的敏感率为 54.2%，对阿莫西林的敏感率为 48.5%，对复方新诺明的敏感率为 28.5%。肠球菌属对头孢曲松敏感的占 61.1%，对克拉维酸敏感的占 33.3%，对氨苄西林敏感的占 27.7%，对阿莫西林和复方新诺明敏感的占 16.6%。链球菌对头孢曲松的敏感率为 83.3%，对氟氯西林的敏感率为 66.6%，对克拉维酸的敏感率为 33.3%，对复方新诺明的敏感率为 16.6%。在革兰氏阴性菌中，发现大肠杆菌对亚胺培南（100%）、头孢曲松（86%）、阿米卡星（100%）、头孢呋辛（73.3%）、氨苄西林（33.3%）、环丙沙星（60%）和庆大霉素（60%）敏感。发现假单胞菌对亚胺培南（50%）、头孢曲松（66.6%）、阿米卡星（83.3%）和头孢呋辛（66.6%）敏感。克雷伯氏菌对亚胺培南（100%）、头孢曲松（75%）、阿米卡星（75%）、头孢呋辛（50%）和环丙沙星（25%）敏感。发现变形杆菌对亚胺培南（100%）、头孢曲松（66.6%）、阿米卡星（66.6%）、头孢呋辛（33.3%）、环丙沙星（66.6%）和庆大霉素（66.6%）敏感。 CBMJ 2024 年 1 月：第 13 卷第 01 期 P: 89-94

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Community Based Medical Journal

自引率

0.00%

发文量