Bacteriological Contamination Level of Foods and Water Sold With Escherichia Coli, Salmonella spp, Staphylococcus Aureus, Coliforms and Vibrio Cholera in Food Establishments in Nairobi City Kenya

Peter Kinyua, Michael Gicheru, Judy Mugo, N. Gitahi
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Abstract

Purpose: Food borne illnesses are major health burden leading to high morbidity and mortality. It is a growing public health concerns worldwide resulting from food and water contaminated by pathogenic microorganism, toxins or chemical hazards. It is estimated that 10 to 20% of food borne illness are contracted from food establishments. The main aim was to determine bacteriological contamination levels of foods and water sold with Escherichia coli, Salmonella spp, Staphylococcus aureus, coliforms and Vibrio cholerae in food establishments in Embakasi South Nairobi City County Kenya. Methodology: The study design was descriptive analytical design. The Samples were collected in selected food establishments (Cafeteria, Hotels, Restaurants and food Kiosks) in Embakasi South Sub county Nairobi City County. The study collected 274 samples of food and water randomly sampled and collected using sterile food bags and water bottles within selected establishments and transported to the laboratory in cool boxes packed with ice packs. The samples were analyzed within 6 hours after collection. Microbiological analysis of food and water were borrowed from WHO and bacteriological analytical manual of foods to identify and isolate coliforms, Staphylococcus aureus, Vibrio cholera, Escherichia coli and Salmonella spp. Results: It was found that some foods and water sold and consumed in the selected food establishments was contaminated with food borne microorganisms. Escherichia coli isolated in 137 food samples were at 24.1%, Vibrio cholera at 23.4%, Staphylococcus aureus at 32.8%, and Salmonella spp at 4.4%. Total coliforms detected in 137 samples of water were at 43.8% where 32.8% of them were Escherichia coli. Unique Contribution to Theory, Practice and Policy: The study concluded there was high level of bacterial contamination of foods and water consumed in selected foods establishments. Nairobi City County health officers should enhance regular sampling of foods and water for microbial quality, health education on sources of food contamination at food eateries and establishments to prevent food and water contamination which later lead to food borne illness outbreak.  
肯尼亚内罗毕市食品店出售的含有大肠杆菌、沙门氏菌、金黄色葡萄球菌、大肠菌群和霍乱弧菌的食品和水的细菌污染水平
目的:食源性疾病是导致高发病率和高死亡率的主要健康负担。由于食物和水受到致病微生物、毒素或化学危害的污染,食源性疾病日益成为全球关注的公共卫生问题。据估计,10% 到 20% 的食源性疾病都是在餐饮场所感染的。这项研究的主要目的是确定肯尼亚内罗毕市南部 Embakasi 县餐饮店出售的食品和饮用水中大肠杆菌、沙门氏菌属、金黄色葡萄球菌、大肠菌群和霍乱弧菌的细菌污染水平。研究方法:研究设计为描述性分析设计。样本从内罗毕市 Embakasi South 子县的选定餐饮场所(自助餐厅、酒店、餐馆和食品亭)采集。该研究随机抽样收集了 274 份食品和水样本,使用无菌食品袋和水瓶在选定的场所内收集,并用装有冰袋的冷藏箱运送到实验室。样本在收集后 6 小时内进行分析。食品和水的微生物分析借鉴了世界卫生组织和《食品细菌分析手册》,以鉴定和分离大肠菌群、金黄色葡萄球菌、霍乱弧菌、大肠杆菌和沙门氏菌:结果发现,在选定的餐饮场所出售和饮用的一些食品和水受到了食源性微生物的污染。在 137 个食品样本中分离出的大肠杆菌占 24.1%,霍乱弧菌占 23.4%,金黄色葡萄球菌占 32.8%,沙门氏菌占 4.4%。在 137 个水样本中检测到的总大肠菌群占 43.8%,其中 32.8%为大肠埃希氏菌。对理论、实践和政策的独特贡献:这项研究得出的结论是,在选定的餐饮场所消费的食品和水的细菌污染程度很高。内罗毕市县卫生官员应加强对食品和水的微生物质量进行定期抽样检查,并在餐饮店和餐饮机构开展有关食品污染源的健康教育,以防止食品和水受到污染,进而导致食源性疾病的爆发。
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