Weitong Zhang, R. Riffiani, K. Sotome, Takayuki Wada, N. Shimomura, S. Boonlue, T. Aimi
{"title":"Comparative Structural Analysis of The Homeodomain Protein in the Bipolar Basidiomycete, Mycoleptodonoides aitchisonii (Bunaharitake)","authors":"Weitong Zhang, R. Riffiani, K. Sotome, Takayuki Wada, N. Shimomura, S. Boonlue, T. Aimi","doi":"10.12982/cmjs.2024.014","DOIUrl":null,"url":null,"abstract":"Mycoleptodonoides aitchisonii is a bipolar mushroom with monokaryons can form clamp cells, regardless of the mating type. Previously, only HD2-18 was identifi ed and characterized at the A locus of Maspi18 (A1). In this study, unlike the A locus in strain Maspi18, HD1-7-like gene (Mahd1-7) and HD2-7 gene (Mahd2-7) were identifi ed at the A locus of the compatible strain Maspi7 (A2), but the nine fl anking genes were highly conserved, suggesting that recombination events may have occurred between HD1 and HD2 genes over evolutionary time. Following phylogenetic and HD protein structural comparisons with other basidiomycetes, homeodomains were identifi ed in HD2-7 and HD2-18, but the former lacked nuclear localization signals (NLS) whereas the latter, like the HD1-7-like protein, did not. These fi ndings indicated that one homeodomain and several NLS on the same or different HD proteins is the minimum suffi cient for driving clamp cell formation in M. aitchisonii. The fi ndings implied that over evolutionary time, unlike HD2, the homeodomain in HD1 does not play a major role in clamp cell formation, and that HD1-7 performs the function of carrying the NLS. Herein, we propose a new hypothesis for clamp cell formation in M. aitchisonii. The fi ndings of this study provide a foundation for understanding the role of heterokaryonization in basidiomycete mushrooms.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2024.014","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Mycoleptodonoides aitchisonii is a bipolar mushroom with monokaryons can form clamp cells, regardless of the mating type. Previously, only HD2-18 was identifi ed and characterized at the A locus of Maspi18 (A1). In this study, unlike the A locus in strain Maspi18, HD1-7-like gene (Mahd1-7) and HD2-7 gene (Mahd2-7) were identifi ed at the A locus of the compatible strain Maspi7 (A2), but the nine fl anking genes were highly conserved, suggesting that recombination events may have occurred between HD1 and HD2 genes over evolutionary time. Following phylogenetic and HD protein structural comparisons with other basidiomycetes, homeodomains were identifi ed in HD2-7 and HD2-18, but the former lacked nuclear localization signals (NLS) whereas the latter, like the HD1-7-like protein, did not. These fi ndings indicated that one homeodomain and several NLS on the same or different HD proteins is the minimum suffi cient for driving clamp cell formation in M. aitchisonii. The fi ndings implied that over evolutionary time, unlike HD2, the homeodomain in HD1 does not play a major role in clamp cell formation, and that HD1-7 performs the function of carrying the NLS. Herein, we propose a new hypothesis for clamp cell formation in M. aitchisonii. The fi ndings of this study provide a foundation for understanding the role of heterokaryonization in basidiomycete mushrooms.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.