Phytochemical Constituents and Potential of Different Extracts from Eleutherine bulbosa on Skin Infectious Treatment : Anti-fungal, Anti-bacterial, Anti-inflammatory and Cytotoxic Activities
{"title":"Phytochemical Constituents and Potential of Different Extracts from Eleutherine bulbosa on Skin Infectious Treatment : Anti-fungal, Anti-bacterial, Anti-inflammatory and Cytotoxic Activities","authors":"Wannisa Raksamat, I. Kimkong","doi":"10.12982/cmjs.2024.002","DOIUrl":null,"url":null,"abstract":"Eleutherine bulbosa is a plant commonly employed in traditional medicine across various tropical regions, including Thailand. The aim of this study was to identify the phytochemical constituents of E. bulbosa, evaluate the inhibitory effects on skin fungal and bacterial pathogens, and examine the anti-inflammatory and cytotoxic properties of extracts obtained from E. bulbosa bulbs through n-Hexane, EtOAc, and EtOH extraction methods. Agar diffusion, MIC, and MBC assays were employed to determine the fungistatic, bacteriostatic, and bactericidal activities. The EtOAc extract of E. bulbosa bulbs demonstrated the highest inhibition against fungal and bacterial strains when compared to other solvents. The results revealed that the EtOAc extract exhibited a potent anti-fungal effect on T. rubrum (the zones of inhibition = 35.00 ± 0.00 mm) comparable to Ketoconazole (the zones of inhibition = 34.33 ± 1.16 mm). The highest antibacterial activity against S. pyogenes was observed, with a zone diameter of 23.00 ± 1.00 mm, while the lowest MIC and MBC values were recorded at 19.53 and 78.12 μg/ml, respectively. Additionally, we demonstrated significant inhibitory properties of E. bulbosa bulbs extracted with n-Hexane on NO production in LPS-stimulated RAW 264.7 cells compared to other solvent extracts. This occurred without affecting cell viability across a concentration range of 6.25 to 25 μg/ml. Moreover, the less affected viability of fibroblasts suggests that the extract may be useful in preventing inflammatory diseases mediated by excessive production of NO with low toxicity to normal cells. Phytochemical screening indicated the presence of various compounds with biological activities including alkaloids, flavonoids, phenols, and tannins in all different solvent extracts. These findings suggest that E. bulbosa bulb extracts possess beneficial effects and hold potential for further development as a new natural source of multifunctional therapeutic agents, which are safe and effective for a broad spectrum of skin infectious microorganisms and also potentially reduce inflammation that is associated with an infection caused by microorganisms.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2024.002","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Eleutherine bulbosa is a plant commonly employed in traditional medicine across various tropical regions, including Thailand. The aim of this study was to identify the phytochemical constituents of E. bulbosa, evaluate the inhibitory effects on skin fungal and bacterial pathogens, and examine the anti-inflammatory and cytotoxic properties of extracts obtained from E. bulbosa bulbs through n-Hexane, EtOAc, and EtOH extraction methods. Agar diffusion, MIC, and MBC assays were employed to determine the fungistatic, bacteriostatic, and bactericidal activities. The EtOAc extract of E. bulbosa bulbs demonstrated the highest inhibition against fungal and bacterial strains when compared to other solvents. The results revealed that the EtOAc extract exhibited a potent anti-fungal effect on T. rubrum (the zones of inhibition = 35.00 ± 0.00 mm) comparable to Ketoconazole (the zones of inhibition = 34.33 ± 1.16 mm). The highest antibacterial activity against S. pyogenes was observed, with a zone diameter of 23.00 ± 1.00 mm, while the lowest MIC and MBC values were recorded at 19.53 and 78.12 μg/ml, respectively. Additionally, we demonstrated significant inhibitory properties of E. bulbosa bulbs extracted with n-Hexane on NO production in LPS-stimulated RAW 264.7 cells compared to other solvent extracts. This occurred without affecting cell viability across a concentration range of 6.25 to 25 μg/ml. Moreover, the less affected viability of fibroblasts suggests that the extract may be useful in preventing inflammatory diseases mediated by excessive production of NO with low toxicity to normal cells. Phytochemical screening indicated the presence of various compounds with biological activities including alkaloids, flavonoids, phenols, and tannins in all different solvent extracts. These findings suggest that E. bulbosa bulb extracts possess beneficial effects and hold potential for further development as a new natural source of multifunctional therapeutic agents, which are safe and effective for a broad spectrum of skin infectious microorganisms and also potentially reduce inflammation that is associated with an infection caused by microorganisms.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.