In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice.

IF 4.4 Q1 IMMUNOLOGY

ImmunoTargets and Therapy Pub Date : 2024-01-23 eCollection Date: 2024-01-01 DOI:10.2147/ITT.S448317

Kassahun Dires Ayenew, Amare Ayalew, Yehualashet Teshome Wondmkun, Abate Wondesen Tsige, Addis Wolde, Yihenew Sewale, Gebremedhin Desta Belihu, Engidashet Fekade, Habtemariam Alekaw Habteweld

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Abstract

Background: Ethiopians use Artemisia abyssinica and Lepidium sativum as immunity enhancers. However, there is no scientific validation conducted so far regarding this claim. The aim of this study was to investigate the in-vivo immunomodulatory activities of essential oils of A. abyssinica and L. sativum in mice.

Methods: The extraction was carried out using the earlier techniques. By hydro distilling fresh seeds and aerial portions of A. abyssinica and L. sativum, respectively, essential oils were obtained. Essential oils of both plants were tested at 100, 200 and 400 mg/kg. The rate of carbon clearance, humoral antibody titer, delayed type hypersensitivity response, spleen and thymus indices were evaluated in mice according to scientific protocols. The carbon clearance assay was determined using carbon ink. Sheep red blood cell was used as an antigen for other tests.

Results: Essential oils of A. abyssinica and L. sativum at 400 mg/kg significantly increased the rate of carbon clearance from the body of mice (p<0.05). The maximum carbon clearance rate was achieved for A. byssinica essential oil at 400 mg/kg. Both essential oils raised the level of HAT to SRBC in comparison to the vehicle and cyclophosphamide administered groups. The largest (84.668±1.951) mean secondary HAT to SRBC was generated by L. sativum essential oil at 400 mg/kg (p<0.001). A. abyssinica essential oil at 200 and 400 mg/kg significantly increased the level of thymus index compared to the model group (p<0.05 and 0.01 respectively). The levamisole group experienced the highest increase in thymus index (p<0.001). Essential oil of L. sativum at 400 mg/kg also increased the level of thymus index. The spleen index in mice was improved by the essential oils only at the highest dose levels (400 mg/kg).

Conclusion: It can be inferred that the essential oils of L. sativum and A. abyssinica have immunostimulant properties.

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阿比西尼亚蒿和莴苣精油在小鼠体内的免疫调节活性

背景：埃塞俄比亚人将阿比西尼亚蒿和莴苣用作免疫增强剂。然而，迄今为止还没有对这一说法进行科学验证。本研究旨在调查阿比西尼亚蒿和莴苣精油在小鼠体内的免疫调节活性：方法：采用早期技术进行提取。方法：采用先前的技术进行萃取，通过水蒸馏分别获得 A. abyssinica 和 L. sativum 的新鲜种子和气生部分的精油。两种植物精油的测试剂量分别为 100、200 和 400 mg/kg。按照科学规程对小鼠的碳清除率、体液抗体滴度、迟发型超敏反应、脾脏和胸腺指数进行了评估。碳清除率测定使用碳墨。其他测试使用绵羊红细胞作为抗原：结果：400 毫克/千克的 A. abyssinica 和 L. sativum 精油能显著提高小鼠体内的碳清除率（pA. byssinica 精油 400 毫克/千克，L. sativum 精油 400 毫克/千克）。与给药组和环磷酰胺给药组相比，两种精油都能提高 HAT 到 SRBC 的水平。与模型组相比，ppL.sativum精油（400 mg/kg）产生的对SRBC的次级HAT平均值最大（84.668±1.951）（ppL.abyssinica精油（200和400 mg/kg）显著提高了胸腺指数水平，ppL.sativum精油（400 mg/kg）也提高了胸腺指数水平）。只有在最高剂量水平（400 毫克/千克）时，小鼠的脾脏指数才会得到精油的改善：结论：可以推断，L. sativum 和 A. abyssinica 的精油具有免疫刺激特性。

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来源期刊

ImmunoTargets and Therapy IMMUNOLOGY-

CiteScore

16.50

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Immuno Targets and Therapy is an international, peer-reviewed open access journal focusing on the immunological basis of diseases, potential targets for immune based therapy and treatment protocols employed to improve patient management. Basic immunology and physiology of the immune system in health, and disease will be also covered.In addition, the journal will focus on the impact of management programs and new therapeutic agents and protocols on patient perspectives such as quality of life, adherence and satisfaction.