Sulfated Chitosan Nanofibrous Scaffolds Seeded With Adipose Stem Cells Promote Ischemic Wound Healing in a Proangiogenic Strategy.

IF 3.2 4区 医学 Q3 CELL & TISSUE ENGINEERING
Xi Zhang, Yan Jiao, Tong Shen, Yuanman Yu, Zhou Yu, Juanli Dang, Lin Chen, Yu Zhang, Guofang Shen
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Abstract

Ischemic wounds are chronic wounds with poor blood supply that delays wound reconstruction. To accelerate wound healing and promote angiogenesis, adipose-derived stem cells (ADSCs) are ideal seed cells for stem cell-based therapies. Nevertheless, providing a favorable environment for cell proliferation and metabolism poses a substantial challenge. A highly sulfated heparin-like polysaccharide 2-N, 6-O-sulfated chitosan (26SCS)-doped poly(lactic-co-glycolic acid) scaffold (S-PLGA) can be used due to their biocompatibility, mechanical properties, and coagent 26SCS high affinity for growth factors. In this study, a nano-scaffold system, constructed from ADSCs seeded on electrospun fibers of modified PLGA, was designed to promote ischemic wound healing. The S-PLGA nanofiber membrane loaded with adipose stem cells ADSCs@S-PLGA was prepared by a co-culture in vitro, and the adhesion and compatibility of cells on the nano-scaffolds were explored. Scanning electron microscopy was used to observe the growth state and morphological changes of ADSCs after co-culture with PLGA electrospun fibers. The proliferation and apoptosis after co-culture were detected using a Cell Counting Kit-8 kit and flow cytometry, respectively. An ischemic wound model was then established, and we further studied the ability of ADSCs@S-PLGA to promote wound healing and angiogenesis. We successfully established ischemic wounds on the backs of rats and demonstrated that electrospun fibers combined with the biological effects of adipose stem cells effectively promoted wound healing and the growth of microvessels around the ischemic wounds. Phased research results can provide a theoretical and experimental basis for a new method for promoting clinical ischemic wound healing.

硫酸化壳聚糖纳米纤维支架在促血管生成策略中播种脂肪干细胞可促进缺血性伤口愈合
缺血性伤口是血液供应不足的慢性伤口,会延迟伤口重建。为了加速伤口愈合和促进血管生成,脂肪源性干细胞(ADSCs)是干细胞疗法的理想种子细胞。然而,为细胞增殖和新陈代谢提供有利环境是一项巨大挑战。由于高度硫酸化的肝素样多糖2-N,6-O-硫酸化壳聚糖(26SCS)掺杂的聚(乳酸-共-乙醇酸)支架(S-PLGA)具有生物相容性、机械性能和助剂26SCS对生长因子的高亲和力,因此可以使用。本研究设计了一种纳米支架系统,该系统由播种在改性聚乳酸(PLGA)电纺纤维上的 ADSCs 构建而成,用于促进缺血性伤口愈合。通过体外共培养制备了负载脂肪干细胞ADSCs@S-PLGA的S-PLGA纳米纤维膜,并探讨了细胞在纳米支架上的粘附性和相容性。扫描电子显微镜观察了 ADSCs 与 PLGA 电纺纤维共培养后的生长状态和形态变化。使用细胞计数试剂盒-8 和流式细胞术分别检测了共培养后 ADSCs 的增殖和凋亡情况。随后,我们建立了缺血伤口模型,并进一步研究了 ADSCs@S-PLGA 促进伤口愈合和血管生成的能力。我们成功地在大鼠背部建立了缺血伤口,并证明电纺纤维与脂肪干细胞的生物效应相结合,能有效促进伤口愈合和缺血伤口周围微血管的生长。阶段性研究成果可为促进临床缺血性伤口愈合的新方法提供理论和实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Transplantation
Cell Transplantation 生物-细胞与组织工程
CiteScore
6.00
自引率
3.00%
发文量
97
审稿时长
6 months
期刊介绍: Cell Transplantation, The Regenerative Medicine Journal is an open access, peer reviewed journal that is published 12 times annually. Cell Transplantation is a multi-disciplinary forum for publication of articles on cell transplantation and its applications to human diseases. Articles focus on a myriad of topics including the physiological, medical, pre-clinical, tissue engineering, stem cell, and device-oriented aspects of the nervous, endocrine, cardiovascular, and endothelial systems, as well as genetically engineered cells. Cell Transplantation also reports on relevant technological advances, clinical studies, and regulatory considerations related to the implantation of cells into the body in order to provide complete coverage of the field.
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