Traumatic brain injury-induced disruption of the circadian clock.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-03-01 Epub Date: 2024-01-29 DOI:10.1007/s00109-024-02416-w

Lu-Ting Kuo, Hsueh-Yi Lu, Yi-Hsing Chen

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Abstract

Disturbances in the circadian rhythm have been reported in patients following traumatic brain injury (TBI). However, the rhythmic expression of circadian genes in peripheral blood leukocytes (PBL) following TBI has not yet been studied. The messenger ribonucleic acid (mRNA) expression of period 1 (Per1), Per2, Per3, cryptochrome 1 (Cry1), Cry2, brain and muscle aryl hydrocarbon receptor nuclear translocator-like 1 (Bmal1), and circadian locomotor output cycles kaput (Clock) was quantified in PBLs from sham-operated rats and rats with acute subdural hematoma (ASDH) over a 48-h period. The rectal temperature of the animals was measured every 4 h over 2 days. The mesor, rhythm, amplitude, and acrophase were estimated using cosinor analysis. Cosinor analysis revealed that Per2, Cry1, and Bmal1 mRNAs were rhythmically expressed in the PBLs of sham-operated rats. In contrast, fluctuations in rhythmic expression were not observed following ASDH. The rectal temperature of sham-operated rats also exhibited rhythmicity. ASDH rats had a disrupted rectal temperature rhythm, a diminished amplitude, and an acrophase shift. TBI with ASDH results in dysregulated expression of some circadian genes and changes in body temperature rhythm. Further research is required to understand the pathophysiology of altered circadian networks following TBI. KEY MESSAGES: First to investigate the mRNA expression of circadian genes in PBLs of ASDH rats. ASDH rats had disrupted rhythmicity of Per2, Cry1, and Bmal1 mRNA expression. Cosinor analysis showed that ASDH rats had a disrupted rectal temperature rhythm.

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脑外伤引起的昼夜节律紊乱。

据报道，脑外伤（TBI）患者的昼夜节律出现紊乱。然而，关于创伤性脑损伤后外周血白细胞（PBL）中昼夜节律基因的节律性表达还没有研究。研究人员对假手术大鼠和急性硬膜下血肿（ASDH）大鼠的外周血白细胞中周期1（Per1）、Per2、Per3、隐色素1（Cry1）、Cry2、脑和肌肉芳基烃受体核转运体样1（Bmal1）以及昼夜节律运动输出周期kaput（Clock）的信使核糖核酸（mRNA）表达进行了48小时的量化。在两天内，每隔 4 小时测量一次动物的直肠温度。采用余弦分析法估算中位数、节律、振幅和倒相。Cosinor分析显示，Per2、Cry1和Bmal1 mRNA在假手术大鼠的PBL中呈节律性表达。相比之下，ASDH术后未观察到节律性表达的波动。假手术大鼠的直肠温度也表现出节律性。ASDH 大鼠的直肠温度节律被打乱，振幅减弱，并出现前相移。带有 ASDH 的创伤性脑损伤会导致一些昼夜节律基因表达失调和体温节律的变化。要了解创伤后昼夜节律网络改变的病理生理学，还需要进一步的研究。关键信息：首次研究 ASDH 大鼠胰岛细胞中昼夜节律基因 mRNA 的表达。ASDH大鼠Per2、Cry1和Bmal1 mRNA表达的节律性被破坏。Cosinor分析表明，ASDH大鼠的直肠温度节律紊乱。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.