Epigenetic changes in pyloric caeca of Atlantic salmon fed diets containing increasing levels of lipids and choline.

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Epigenetics Pub Date : 2024-12-01 Epub Date: 2024-01-28 DOI:10.1080/15592294.2024.2305079
Anusha K S Dhanasiri, Daphne Siciliani, Trond M Kortner, Åshild Krogdahl
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引用次数: 0

Abstract

An earlier study of ours investigating the effect of dietary lipid levels on the choline requirement of Atlantic salmon showed increasing severity of intestinal steatosis with increasing lipid levels. As choline is involved in epigenetic regulation by being the key methyl donor, pyloric caeca samples from the study were analysed for epigenetic effects of dietary lipid and choline levels. The diets varied in lipid levels between 16% and 28%, and choline levels between 1.9 and 2.3 g/kg. The diets were fed for 8 weeks to Atlantic salmon of 25 g of initial weight. Using reduced representation bisulfite sequencing (RRBS), this study revealed that increasing dietary lipid levels induced methylation differences in genes involved in membrane transport and signalling pathways, and in microRNAs important for the regulation of lipid homoeostasis. Increasing choline levels also affected genes involved in fatty acid biosynthesis and transport, lipolysis, and lipogenesis, as well as important immune genes. Our observations confirmed that choline is involved in epigenetic regulation in Atlantic salmon, as has been reported for higher vertebrates. This study showed the need for the inclusion of biomarkers of epigenetic processes in studies that must be conducted to define optimal choline levels in diets for Atlantic salmon.

大西洋鲑幽门盲肠的表观遗传学变化,喂食含脂量和胆碱量不断增加的食物。
我们早先的一项研究调查了膳食脂质水平对大西洋鲑胆碱需求量的影响,结果显示,随着脂质水平的增加,肠道脂肪变性的严重程度也在增加。由于胆碱作为关键的甲基供体参与了表观遗传调节,因此我们对研究中的幽门盲肠样本进行了分析,以了解膳食脂质和胆碱水平对表观遗传的影响。日粮中的脂质含量在 16% 到 28% 之间,胆碱含量在 1.9 到 2.3 克/千克之间。给初始体重为 25 克的大西洋鲑鱼喂食这些日粮 8 周。这项研究利用还原表征亚硫酸氢盐测序(RRBS)发现,膳食脂质水平的增加会诱导参与膜运输和信号通路的基因以及对调节脂质平衡很重要的微RNA的甲基化差异。胆碱水平的增加也会影响参与脂肪酸生物合成和运输、脂肪分解和脂肪生成的基因以及重要的免疫基因。我们的观察结果证实,胆碱参与了大西洋鲑的表观遗传调控,这在高等脊椎动物中已有报道。这项研究表明,有必要在研究中纳入表观遗传过程的生物标志物,以确定大西洋鲑鱼日粮中的最佳胆碱水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Epigenetics
Epigenetics 生物-生化与分子生物学
CiteScore
6.80
自引率
2.70%
发文量
82
审稿时长
3-8 weeks
期刊介绍: Epigenetics publishes peer-reviewed original research and review articles that provide an unprecedented forum where epigenetic mechanisms and their role in diverse biological processes can be revealed, shared, and discussed. Epigenetics research studies heritable changes in gene expression caused by mechanisms others than the modification of the DNA sequence. Epigenetics therefore plays critical roles in a variety of biological systems, diseases, and disciplines. Topics of interest include (but are not limited to): DNA methylation Nucleosome positioning and modification Gene silencing Imprinting Nuclear reprogramming Chromatin remodeling Non-coding RNA Non-histone chromosomal elements Dosage compensation Nuclear organization Epigenetic therapy and diagnostics Nutrition and environmental epigenetics Cancer epigenetics Neuroepigenetics
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