Analysis of the Expression of PRDX6 in Patients with Hepatocellular Carcinoma and its Effect on the Phenotype of Hepatocellular Carcinoma Cells

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current Genomics Pub Date : 2024-01-23 DOI:10.2174/0113892029273682240111052317

Mu Runhong, Chang Mingzhu, Feng Chuanbo, Cui Yunhe, Li Tingyu, Liu Chang, Wang Yilin, Guo Xiao

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引用次数: 0

Abstract

Objective:: This research aimed to study the expression of PRDX6 mRNA in hepatocellular carcinoma (HCC) and its effect on the prognosis of HCC. Moreover, the effect of PRDX6 gene knockdown on the proliferation, migration, and invasion of HepG2 cells mediated by lentivirus was also examined. This study offers a theoretical and experimental basis for further research on the mechanism of PRDX6 in liver cancer and new methods for clinical diagnosis and treatment. Methods:: RNA sequence data of 369 HCC patients were screened through the TCGA database, and the expression and clinical characteristics of PRDX6 mRNA were analyzed based on high- -throughput RNA sequencing data. HepG2 cells were divided into WT, sh-NC and sh-PRDX6 groups. Real-time PCR and Western blot were used to detect the expression levels of the PRDX6 gene and protein, respectively. CCK8 method was used to detect the proliferation activity of Hep- G2 cells, scratch healing test was used to detect the migration ability, Transwell chamber was used to detect the invasion ability, and Western blot was used to detect the expression levels of PI3K/Akt/mTOR signaling pathway and Notch signaling pathway-related proteins. Results:: The expression of PRDX6 was significantly correlated with the gender, race, clinical stage, histological grade, and survival time of HCC patients (P < 0.05). Compared with that in WT and sh-NC groups, the expression level of PRDX6 protein in HCC patients was significantly lower (P < 0.01), the proliferation activity of HCC cells was significantly decreased (P < 0.05), and the migration and invasion ability was significantly decreased (P <0.05) in the sh-PRDX6 group. The expression levels of PI3K, p-Akt, p-mTOR, Notch1, and Hes1 proteins in the sh- PRDX6 group were significantly lower than those in WT and sh-NC groups (P < 0.05). Conclusion:: The expression of PRDX6 may be closely related to the prognosis of HCC. Lentivirus- mediated PRDX6 knockdown can inhibit the proliferation, migration and invasion of HCC cells, which may be related to its regulating the PI3K/Akt/mTOR and Notch1 signaling pathways. PRDX6 is expected to be a new target for the diagnosis and treatment of liver cancer.

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肝细胞癌患者体内 PRDX6 的表达及其对肝细胞癌细胞表型的影响分析

研究目的本研究旨在探讨 PRDX6 mRNA 在肝细胞癌（HCC）中的表达及其对 HCC 预后的影响。此外，还研究了慢病毒介导的 PRDX6 基因敲除对 HepG2 细胞增殖、迁移和侵袭的影响。本研究为进一步研究 PRDX6 在肝癌中的作用机制以及临床诊断和治疗的新方法提供了理论和实验依据。研究方法通过 TCGA 数据库筛选了 369 例 HCC 患者的 RNA 序列数据，基于高通量 RNA 测序数据分析了 PRDX6 mRNA 的表达和临床特征。将 HepG2 细胞分为 WT 组、sh-NC 组和 sh-PRDX6 组。实时 PCR 和 Western 印迹法分别检测 PRDX6 基因和蛋白的表达水平。CCK8法检测Hep- G2细胞的增殖活性，划痕愈合试验检测迁移能力，Transwell室检测侵袭能力，Western blot检测PI3K/Akt/mTOR信号通路和Notch信号通路相关蛋白的表达水平。结果PRDX6的表达与HCC患者的性别、种族、临床分期、组织学分级和生存时间显著相关（P< 0.05）。与 WT 组和 sh-NC 组相比，sh-PRDX6 组 HCC 患者的 PRDX6 蛋白表达水平明显降低（P <0.01），HCC 细胞的增殖活性明显降低（P <0.05），迁移和侵袭能力明显降低（P <0.05）。sh- PRDX6组PI3K、p-Akt、p-mTOR、Notch1和Hes1蛋白的表达水平明显低于WT组和sh-NC组（P <0.05）。结论：：PRDX6的表达可能与HCC的预后密切相关。慢病毒介导的 PRDX6 基因敲除可抑制 HCC 细胞的增殖、迁移和侵袭，这可能与其调节 PI3K/Akt/mTOR 和 Notch1 信号通路有关。PRDX6有望成为诊断和治疗肝癌的新靶点。

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来源期刊

Current Genomics 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Current Genomics is a peer-reviewed journal that provides essential reading about the latest and most important developments in genome science and related fields of research. Systems biology, systems modeling, machine learning, network inference, bioinformatics, computational biology, epigenetics, single cell genomics, extracellular vesicles, quantitative biology, and synthetic biology for the study of evolution, development, maintenance, aging and that of human health, human diseases, clinical genomics and precision medicine are topics of particular interest. The journal covers plant genomics. The journal will not consider articles dealing with breeding and livestock. Current Genomics publishes three types of articles including: i) Research papers from internationally-recognized experts reporting on new and original data generated at the genome scale level. Position papers dealing with new or challenging methodological approaches, whether experimental or mathematical, are greatly welcome in this section. ii) Authoritative and comprehensive full-length or mini reviews from widely recognized experts, covering the latest developments in genome science and related fields of research such as systems biology, statistics and machine learning, quantitative biology, and precision medicine. Proposals for mini-hot topics (2-3 review papers) and full hot topics (6-8 review papers) guest edited by internationally-recognized experts are welcome in this section. Hot topic proposals should not contain original data and they should contain articles originating from at least 2 different countries. iii) Opinion papers from internationally recognized experts addressing contemporary questions and issues in the field of genome science and systems biology and basic and clinical research practices.