Euryhaline copepod Pseudodiaptomus inopinus changed the prey preference of red sea bream Pagrus major larvae

Abstract

We evaluated the potential of the euryhaline copepod Pseudodiaptomus inopinus as a prey to enhance the feeding activity of red sea bream Pagrus major larvae. Pseudodiaptomus inopinus was used to evaluate free amino acid composition and dietary effects on the rearing performance of fish larvae, in comparison to rotifers (control prey). Among free amino acids, alanine, arginine, and glycine were markedly higher in P. inopinus than in rotifers. Larvae were reared for 20 days post-hatching under three feeding treatments: rotifers (control), rotifers supplemented with copepods, and copepods only. Larvae fed copepods alone had a higher growth rate than those in the other treatments. However, the survival rates of these larvae were lower than those under control or copepod supplementation. While equivalent stocking densities of rotifers were employed in the rearing water for both larvae, whether supplemented with copepods or not, the supplemented treatment yielded diminished larval survival and failed to enhance growth rates. According to gut content analysis, larvae receiving supplemented treatment preferentially fed on copepods, and the number of ingested rotifers was reduced. As a result, total ingested mass was lower in the supplemented larvae. This study suggests that copepods containing free amino acid species negatively affect prey acceptance of fish larvae.