Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin

IF 1.5 3区农林科学 Q2 AGRONOMY

Phytoparasitica Pub Date : 2024-01-25 DOI:10.1007/s12600-024-01122-0

Izaías Araújo de Oliveira, Luciane de Nazaré Almeida dos Reis, Flávia Milene Barros Nery, Niday Alline Nunes Fernandes, Vinícius Peres Machado Reis Gonçalves, Maria R. Rojas, Robert L. Gilbertson, Ailton Reis, Leonardo Silva Boiteux, Maria Esther de Noronha Fonseca-Boiteux, Rita de Cássia Pereira-Carvalho

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Abstract

Foliar samples were collected from the tomato (Solanum lycopersicum) cultivar ‘IPA-6’ exhibiting begomovirus-like symptoms (apical leaf curling and chlorosis) in Iranduba (Brazilian Amazon). PCR tests with degenerate primers targeting DNA–A and DNA–B components of a sample designated as AM–035 were positive for both genomic regions. Full-length cognate DNA–A and DNA–B components of AM–035 were cloned and sequenced via Sanger dideoxy termination reaction. The complete sequence of the DNA–A component (MK878452) comprised 2,630 nucleotides (organized in five open reading frames – ORFs), and the DNA–B component (MK878451) displayed 2,593 nucleotides (two ORFs). The highest identities ranged from 90 to 92% with tomato chlorotic mottle Guyane virus (ToCMoGV) isolates from French Guiana, indicating a highly divergent Brazilian strain of this virus. The present work reinforces the scenario of the high diversity of tomato-infecting begomoviruses in Brazil. Further studies are needed to determine the distribution and prevalence of ToCMoGV across tomato-producing areas in the Brazilian Amazon River Basin.

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巴西亚马逊河流域高度分化的番茄萎黄斑驳盖因病毒株的基因组特征

在伊兰杜巴（巴西亚马逊河流域）采集了番茄（Solanum lycopersicum）栽培品种 "IPA-6 "的叶片样本，该品种表现出类似乞猴病毒的症状（叶片顶端卷曲和萎黄）。使用针对 DNA-A 和 DNA-B 组成部分的退化引物对 AM-035 样品进行 PCR 检测，结果显示两个基因组区域均呈阳性。通过 Sanger 双脱氧终止反应，克隆了 AM-035 的全长同源 DNA-A 和 DNA-B 组份，并对其进行了测序。DNA-A成分（MK878452）的完整序列包括2,630个核苷酸（由五个开放阅读框（ORF）组成），DNA-B成分（MK878451）的完整序列包括2,593个核苷酸（两个ORF）。与来自法属圭亚那的番茄萎黄斑驳病病毒（ToCMoGV）分离物的最高相同度为 90% 至 92%，表明该病毒的巴西株高度分化。本研究结果进一步证实了巴西番茄感染乞猴病毒的高度多样性。要确定 ToCMoGV 在巴西亚马逊河流域番茄产区的分布和流行情况，还需要进一步的研究。

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来源期刊

Phytoparasitica 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytoparasitica is an international journal on Plant Protection, that publishes original research contributions on the biological, chemical and molecular aspects of Entomology, Plant Pathology, Virology, Nematology, and Weed Sciences, which strives to improve scientific knowledge and technology for IPM, in forest and agroecosystems. Phytoparasitica emphasizes new insights into plant disease and pest etiology, epidemiology, host-parasite/pest biochemistry and cell biology, ecology and population biology, host genetics and resistance, disease vector biology, plant stress and biotic disorders, postharvest pathology and mycotoxins. Research can cover aspects related to the nature of plant diseases, pests and weeds, the causal agents, their spread, the losses they cause, crop loss assessment, and novel tactics and approaches for their management.