Fatima Diya, Sylvain Benhamou, Antonio Biondi, Nicolas Desneux, Isabelle Rahioui, Agnes Vallier, Catherine Sivignon, Francine Rizk, Linda Kfoury, Pedro Da Silva
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引用次数: 0
Abstract
Tuta absoluta, one of the most damaging pests of tomato crops and other solanaceous plants, has been extensively managed by the use of synthetic pesticides. Unfortunately, the reliance on chemicals has negatively affected human health, biodiversity, and the environment. Therefore, the development of eco-friendly alternatives is highly encouraged to replace hazardous chemical pesticides. Inhibitory cystine knot (ICK) peptides have a leading future as they present a narrow spectrum activity, and remarkable stability, making them promising bio-insecticide modalities against pests of crops of economic importance. In this study, the impact of Pea albumin 1-b (PA1b), a plant ICK peptide extracted from seeds of peas (Pisum sativum, Fabaceae) was investigated through foliar application to be delivered to 2nd instar larvae of T. absoluta. However, due to the failure of delivery upon foliar application, microinjections of the peptide into 3rd instar larvae were performed to assess the insecticidal activity of PA1b when being into the body cavity of the larvae. Toxicity bioassays were conducted in Petri dishes in a developed experimental arena that allowed us to easily survey the status of the larva and the leaf for up to 18 days. Our study showed that PA1b has a significant toxic effect against T. absoluta when delivered through injection. Further studies must be conducted to optimize the administration of PA1b into the larvae of T. absoluta, investigate the binding sites of the peptide in this pest, and ascertain the insecticidal capacity of such a peptide at an agricultural-relevant scale.
期刊介绍:
Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology.
Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods.
Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.