Therapeutic eﬀects of a novel venom abstract (ZK002) solution in an alkali-burned corneal wound-healing model.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2023-12-15 eCollection Date: 2023-01-01

Wen-Yan Peng, Fei Wang, Shuang-Jian Yang, Qin-Yan Sun, Heng-Shen Zhou, Xiaoyi Li, Zheng-Xuan Jiang, Shi-You Zhou

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引用次数: 0

Abstract

Purpose: Corneal alkali burns can progress to corneal epithelial defects, inflammation, scarring, and angiogenesis, potentially leading to blindness. Therefore, we examined the therapeutic effects of a novel ophthalmic solution (ZK002) on wound healing in alkali-burned rat corneas.

Methods: In this study, we attempted to treat alkali-exposed rat corneas using topical application of either an ophthalmic solution with ZK002 or an anti-vascular endothelial growth factor agent for 14 days. We evaluated corneal edema, corneal neovascularization area, and histological changes. We also assessed the inflammatory (MMP-9, MMP-2, and interleukin-1β) and angiogenic (vascular endothelial growth factor receptor 2, VEGFR2) markers. Levels of inflammatory (matrix metalloproteinase (MMP)-9, MMP-2, and interleukin-1β), profibrotic (α-smooth muscle actin, α-SMA; transforming growth factor-β2,TGF-β2), and angiogenic (vascular endothelial growth factor-receptor 2, VEGFR2) factors, as well as peroxisome proliferator-activated receptor γ (PPARγ) mRNA expression, were measured.

Results: The analyses showed that alkali exposure caused an increase in corneal edema and fibrosis with corneal neovascularization. The accumulation of α-smooth muscle actin-positive myofibroblasts and the deposition of transforming growth factor-β2 on the alkali-exposed corneas were noted on day 14. The mRNA expression levels of interleukin-1β, MMP-9, MMP-2, VEGFR2, and profibrotic factors were decreased in the ZK002 group compared with the control group during the early period of corneal alkali burns on day 14. However, the expression level of PPARγ mRNA was increased in the ZK002 group.

Conclusions: ZK002 decreased the fibrotic reaction and prevented neovascularization in the cornea after an alkali burn. Therefore, the novel ophthalmic solution ZK002 could be a potentially promising therapeutic clinical treatment for corneal wound healing.

本刊更多论文

新型毒液摘要（ZK002）溶液在碱烧伤角膜伤口愈合模型中的治疗效果。

目的：角膜碱烧伤可发展为角膜上皮缺损、炎症、瘢痕和血管生成，有可能导致失明。因此，我们研究了一种新型眼科溶液（ZK002）对碱烧伤大鼠角膜伤口愈合的治疗效果：在这项研究中，我们尝试使用含 ZK002 的眼药水或抗血管内皮生长因子药剂对碱暴露的大鼠角膜进行为期 14 天的局部治疗。我们对角膜水肿、角膜新生血管面积和组织学变化进行了评估。我们还评估了炎症指标（MMP-9、MMP-2 和白细胞介素-1β）和血管生成指标（血管内皮生长因子受体 2，VEGFR2）。测量了炎症因子（基质金属蛋白酶 (MMP)-9、MMP-2 和白细胞介素-1β）、坏死因子（α-平滑肌肌动蛋白，α-SMA；转化生长因子-β2，TGF-β2）和血管生成因子（血管内皮生长因子受体 2，VEGFR2）的水平以及过氧化物酶体增殖激活受体 γ（PPARγ）mRNA 的表达：分析表明，碱暴露导致角膜水肿和纤维化加重，并伴有角膜新生血管。第 14 天，受碱暴露的角膜上出现了 α-平滑肌肌动蛋白阳性肌成纤维细胞的聚集和转化生长因子-β2 的沉积。与对照组相比，ZK002 组在第 14 天角膜碱烧伤早期的白细胞介素-1β、MMP-9、MMP-2、血管内皮生长因子受体 2 和组织坏死因子的 mRNA 表达水平有所下降。然而，ZK002组中PPARγ mRNA的表达水平升高：结论：ZK002 能减轻碱烧伤后角膜的纤维化反应并阻止新生血管生成。因此，新型眼药水 ZK002 有可能成为一种治疗角膜伤口愈合的临床疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.