Association of Mitochondrial Pyruvate Carrier with the Clinical and Histological Features in Lupus Nephritis.

IF 2.1 Q2 UROLOGY & NEPHROLOGY
Huanhuan Zhu, Chen Chen, Luhan Geng, Qing Li, Chengning Zhang, Lin Wu, Bo Zhang, Suyan Duan, Changying Xing, Yanggang Yuan
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引用次数: 0

Abstract

Background: Mounting evidence suggests that mitochondrial dysfunction contributes to lupus nephritis (LN) pathogenesis. Mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2) mediating pyruvate transport from the cytoplasm to the mitochondrial matrix, determines the cell survival and cellular energy supply. Here, we aimed to investigate the association of mitochondrial pyruvate carrier expression with the clinical and histological features in LN.

Methods: Patients with biopsy-proven proliferative LN (class III and class IV, n=18) and membranous LN (class V, n=18) were included. Expression of MPC1 and MPC2 were examined by immunohistochemistry. MPC protein levels in the two groups were evaluated by the Student's t-test. Correlation analysis between MPC levels and clinicopathological features was performed by Spearman's rank correlation.

Results: Both MPC1 and MPC2 were exclusively expressed in renal tubules of enrolled LN. Significantly lower MPC1 and MPC2 were observed in patients with proliferative LN compared to membranous LN. In addition, the MPC1 and MPC2 were negatively correlated with SLEDAI-2K score, renal function, and renal pathology activity index.

Conclusion: Both MPC1 and MPC2 were localized in renal tubules, and decreased MPC content was more pronounced in proliferative LN than membranous LN. MPC levels were significantly correlated with renal functions and renal pathology activity.

线粒体丙酮酸载体与狼疮性肾炎临床和组织学特征的关系
背景:越来越多的证据表明,线粒体功能障碍是狼疮性肾炎(LN)的发病机制之一。线粒体丙酮酸载体 1(MPC1)和线粒体丙酮酸载体 2(MPC2)介导丙酮酸从细胞质向线粒体基质的转运,决定着细胞的存活和能量供应。在此,我们旨在研究线粒体丙酮酸载体的表达与 LN 的临床和组织学特征之间的关联:方法:纳入活检证实的增生性 LN(III 级和 IV 级,18 人)和膜性 LN(V 级,18 人)患者。通过免疫组化检查MPC1和MPC2的表达。两组的 MPC 蛋白水平采用学生 t 检验。MPC水平与临床病理特征之间的相关性分析采用Spearman秩相关法进行:结果:MPC1和MPC2均只在入组LN的肾小管中表达。与膜状 LN 相比,增生性 LN 患者的 MPC1 和 MPC2 水平明显较低。此外,MPC1和MPC2与SLEDAI-2K评分、肾功能和肾脏病理活动指数呈负相关:结论:MPC1和MPC2都定位于肾小管,增殖性LN的MPC含量下降比膜性LN更明显。MPC水平与肾功能和肾病变活动度有明显相关性。
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来源期刊
CiteScore
3.90
自引率
5.00%
发文量
40
审稿时长
16 weeks
期刊介绍: International Journal of Nephrology and Renovascular Disease is an international, peer-reviewed, open-access journal focusing on the pathophysiology of the kidney and vascular supply. Epidemiology, screening, diagnosis, and treatment interventions are covered as well as basic science, biochemical and immunological studies. In particular, emphasis will be given to: -Chronic kidney disease- Complications of renovascular disease- Imaging techniques- Renal hypertension- Renal cancer- Treatment including pharmacological and transplantation- Dialysis and treatment of complications of dialysis and renal disease- Quality of Life- Patient satisfaction and preference- Health economic evaluations. The journal welcomes submitted papers covering original research, basic science, clinical studies, reviews & evaluations, guidelines, expert opinion and commentary, case reports and extended reports. The main focus of the journal will be to publish research and clinical results in humans but preclinical, animal and in vitro studies will be published where they shed light on disease processes and potential new therapies and interventions.
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