Daya Hambat Ekstrak Daun Mangrove Bruguiera gymnorrhiza Terhadap Pertumbuhan Candida Albicans pada Gigi Tiruan Lepasan Akrilik

e-GiGi Pub Date : 2024-01-20 DOI:10.35790/eg.v12i2.51335

Johanna A. Khoman, Vonny N. S. Wowor, Billinda B. Tampie

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Abstract

Abstract: Rough surface and poorly kept denture, especially those facing the supporting mucosa, can become a reservoir for the growth of microorganisms, such as the Candida albicans. Natural ingredients are chosen for denture cleanser because they are relatively cheap and safe. The mangrove plant Bruguiera gymnorrhiza is a natural source which contains active compounds, including saponins, flavonoids, phenol hydroquinones, alkaloids, and tannins which are antioxidants, anti-inflammatory, antibacterial, antiviral, antifungal, hypoallergenic, etc. This study aimed to determine the inhibition of Bruguiera gymnorrhiza mangrove leaf extract on the growth of Candida albicans on removable denture plates and to determine the effective concentration that could inhibit the growth of Candida albicans. This was a true experimental laboratory study with a post test control group design. The test method used was the modified Kirby-Bauer method using wells. Samples were divided into four groups with respective concentrations of 20%, 30%, 40%, 50%, control positive, and control negative. The one-way ANOVA test showed a p-value of <0.05, meaning that there was a significant difference in the average inhibition based on the four concentration groups. The Post Hoc test (Tukey's HSD) showed that there were differences in the strength of inhibition at each treatment concentration and the control group. In conclusion, Bruguiera gymnorrhiza mangrove leaf extract can inhibit the growth of the Candida albicans. Keywords: denture stomatitis; Candida albicans; Bruguiera gymnorrhiza Abstrak: Permukaan gigi tiruan yang kasar dan kurang dijaga kebersihannya, terlebih khusus yang berhadapan dengan mukosa pendukung dapat menjadi reservoir bagi pertumbuhan mikroorganisme, seperti jamur Candida albicans yang dapat menyebabkan denture stomatitis. Bahan pembersih alami banyak dipilih karena relatif murah, dan aman. Tanaman mangrove Bruguiera gymnorrhiza merupakan salah satu sumber alami yang memiliki kandungan senyawa aktif, antara lain, saponin, flavonoid, fenol, alkaloid, dan tanin yang bersifat antioksidan, antiinflamasi, antibakteri, antivirus, antifungi, dan antialergi. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun mangrove Bruguiera gymnorrhiza terhadap pertumbuhan Candida albicans pada plat gigi tiruan lepasan dan untuk mengetahui konsentrasi efektif yang dapat menghambat pertumbuhan Candida albicans. Jenis penelitian ialah eksperimental laboratorik murni dengan post test control group design. Metode pengujian yang digunakan yaitu metode modifikasi Kirby-Bauer menggunakan sumuran. Sampel dibagi dalam kelompok perlakuan dengan konsentrasi masing-masing 20%, 30%, 40%, 50%, kontrol positif, dan kontrol negatif. Uji one way ANOVA menunjukkan p<0,05, berarti terdapat perbedaan bermakna untuk rerata daya hambat berdasarkan keempat kelompok konsentrasi. Uji Post Hoc (Tukey’s HSD) menunjukkan adanya perbedaan kekuatan daya hambat pada setiap konsentrasi perlakuan dan kelompok kontrol. Simpulan penelitian ini ialah ekstrak daun mangrove Bruguiera gymnorrhiza dapat menghambat pertumbuhan jamur Candida albicans. Kata kunci: gigi tiruan; denture stomatitis; Candida albicans; Bruguiera gymnorrhiza

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Bruguiera gymnorrhiza 红树林叶提取物对白色念珠菌在丙烯酸活动义齿上生长的抑制作用

摘要：粗糙的义齿表面和保管不善的义齿，尤其是面对支持粘膜的义齿，会成为白色念珠菌等微生物生长的温床。选择天然成分作为义齿清洁剂是因为它们相对便宜且安全。红树植物 Bruguiera gymnorrhiza 是一种天然来源，含有活性化合物，包括皂苷、黄酮类、酚氢醌、生物碱和单宁酸，具有抗氧化、消炎、抗菌、抗病毒、抗真菌和低过敏性等作用。本研究旨在确定 Bruguiera gymnorrhiza 红树叶提取物对活动义齿板上白色念珠菌生长的抑制作用，并确定能抑制白色念珠菌生长的有效浓度。这是一项真正的实验室实验研究，采用试验后对照组设计。使用的测试方法是改良柯比鲍尔法（Kirby-Bauer method）。样品分为四组，浓度分别为 20%、30%、40%、50%，对照组为阳性，对照组为阴性。单因素方差分析检验显示，P 值小于 0.05，即四组浓度的平均抑制率有显著差异。事后检验（Tukey's HSD）表明，各处理浓度组与对照组的抑制强度存在差异。关键词：义齿口腔炎；白色念珠菌；Bruguiera gymnorrhiza Abstrak：在中国，有许多地方都存在着口腔溃疡，其中最常见的是白念珠菌性口腔炎。这些生物的数量众多，而且关系密切。红树林蕴含丰富的抗菌素、皂苷、黄酮类、酚类、生物碱和鞣质，具有抗菌、抗炎、抗溃疡、抗病毒、抗真菌和抗细菌的作用。它的作用是在红树林的鳞茎上种植白念珠菌，并在白念珠菌的鳞茎上建立一个有效的生长中心。实验方法为实验室实验，对照组为试验后设计。采用的实验方法是柯比-鲍尔改良方法。从每组的 20%、30%、40%、50%、阳性对照组和阴性对照组中得出结论。单因素方差分析结果表明，P<0.05 的结果显示了对不同剂量组的日产量的影响。后验差异法（Tukey's HSD）显示，在每组中心和对照组中，日处理量的差异都很大。其研究结果表明，红树林布鲁圭亚丛枝属植物能抑制白色念珠菌的生长。相关资料：鹅口疮；义齿口腔炎；白色念珠菌；布鲁圭亚丛枝属植物

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