A custom 3D printed paddlewheel improves growth in flat panel photobioreactor

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Michelle Meagher, Jacob Tamburro, Nanette R. Boyle
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引用次数: 0

Abstract

One of the main challenges with using flat panel photobioreactors for algal growth is uneven mixing and settling of cells in corners, especially when bubbling is the only method used for mixing. In order to improve mixing in our flat panel reactor, we designed a custom paddlewheel. Paddlewheels are frequently used in outdoor algae raceway ponds to improve mixing and we are taking advantage of the same principle for mixing in the reactor. The paddlewheel is easily integrated into our PSI FMT150 1-L flat panel photobioreactor and is printed on a 3D printer using high temperature poly lactic acid (HT-PLA). With the inclusion of an annealing step, the paddlewheel is autoclavable. Addition of the paddlewheel in the reactor minimized cell settling and improved algal growth, as evidenced by a nearly 40% increase in oxygen production rates. Nutrient dispersion and utilization in the culture was also improved as evidenced by a corresponding 38% decrease in CO2 concentration. The paddlewheel device presented here is a cost-effective method for improving algal growth in a flat panel photobioreactor.

定制的 3D 打印桨轮改善了平板光生物反应器的生长。
使用平板光生物反应器进行藻类生长所面临的主要挑战之一是混合不均和细胞沉淀在角落里,尤其是在只使用气泡进行混合的情况下。为了改善平板反应器的混合效果,我们设计了一个定制的桨轮。桨轮常用于室外水藻赛道池塘,以改善混合效果,我们正在利用同样的原理在反应器中进行混合。桨轮很容易集成到我们的 PSI FMT150 1-L 平板光生物反应器中,并使用高温聚乳酸 (HT-PLA) 在 3D 打印机上打印出来。由于加入了退火步骤,桨轮可高压灭菌。在反应器中添加桨轮可最大限度地减少细胞沉降,改善藻类生长,氧气生产率提高了近 40%。营养物质在培养过程中的分散和利用也得到了改善,二氧化碳浓度相应降低了 38% 就是证明。本文介绍的桨轮装置是一种在平板光生物反应器中改善藻类生长的经济有效的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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