MlrA, an Essential Enzyme for Microcystins and Nodularin on First Step Biodegradation in Microcystin-Degrading Bacteria

IF 3.8 3区医学 Q2 CHEMISTRY, MEDICINAL

Chemical Research in Toxicology Pub Date : 2024-01-22 DOI:10.1021/acs.chemrestox.3c00341

Tianxiao Liu, Narumi Kurogo, Miwa Kodato, Naoshi Fujimoto, Tomoaki Itayama, Kunihiro Okano, Motoo Utsumi, Zhenya Zhang, Zhongfang Lei, Tian Yuan, Hideaki Maseda, Norio Sugiura and Kazuya Shimizu*,

引用次数: 0

Abstract

Microcystin-degrading bacteria first degrade microcystins by microcystinase A (MlrA) to cleave the cyclic structure of microcystins at the Adda-Arg site of microcystin-LR, microcystin-RR, and microcystin-YR, but the cleavage of the other microcystins was not clear. In our study, the microcystin-degrading bacterium Sphingopyxis sp. C-1 as wild type and that of mlrA-disrupting mutant, Sphingopyxis sp. CMS01 were used for microcystins biodegradation. The results showed MlrA degraded microcystin-LA, microcystin-LW, microcystin-LY, microcystin-LF, and nodularin. MlrA could cleave the Adda-L-amino acid site.

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MlrA是微囊藻毒素和Nodularin在微囊藻毒素降解细菌中进行第一步生物降解的必需酶。

降解微囊藻毒素的细菌首先通过微囊藻毒素酶 A（MlrA）裂解微囊藻毒素-LR、微囊藻毒素-RR 和微囊藻毒素-YR 的 Adda-Arg 位点上的环状结构，但对其他微囊藻毒素的裂解尚不清楚。我们的研究以微囊藻毒素降解菌 Sphingopyxis sp.结果表明，MlrA 可降解微囊藻毒素-LA、微囊藻毒素-LW、微囊藻毒素-LY、微囊藻毒素-LF 和球藻毒素。MlrA能裂解Adda-L-氨基酸位点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Research in Toxicology 医学-毒理学

CiteScore

7.90

自引率

7.30%

发文量

215

审稿时长

3.5 months

期刊介绍： Chemical Research in Toxicology publishes Articles, Rapid Reports, Chemical Profiles, Reviews, Perspectives, Letters to the Editor, and ToxWatch on a wide range of topics in Toxicology that inform a chemical and molecular understanding and capacity to predict biological outcomes on the basis of structures and processes. The overarching goal of activities reported in the Journal are to provide knowledge and innovative approaches needed to promote intelligent solutions for human safety and ecosystem preservation. The journal emphasizes insight concerning mechanisms of toxicity over phenomenological observations. It upholds rigorous chemical, physical and mathematical standards for characterization and application of modern techniques.