Ameliorative effects of Bifidobacterium longum peptide-1 on benzo(α)pyrene induced oxidative damages via daf-16 in Caenorhabditis elegans.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Ling Ai, Dan Luo, Huailing Wang, Xiaoyu Liu, Min Yang, Fangfang Tian, Suofu Qin, Jie Liu, Yuying Li
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Abstract

Oxidative stress is implicated in numerous diseases, with benzo(α)pyrene (BaP) known for causing substantial oxidative damage. Bifidobacterium longum (B. longum) is recognized as an antioxidant bacterium for certain hosts, yet its influence on oxidative damages instigated by BaP remains undetermined. In our study, we introduced various strains of Caenorhabditis elegans (C. elegans) to BaP to trigger oxidative stress, subsequently treating them with different forms of B. longum to evaluate its protective effects. Additionally, we explored the role of daf-16 in this context. Our findings indicated that in wild-type N2 C. elegans, B. longum-even in the form of inactivated bacteria or bacterial ultrasonic lysates (BULs)-significantly extended lifespan. BaP exposure notably decreased lifespan, superoxide dismutase (SOD) activity, and motility, while simultaneously down-regulating the expression of reactive oxygen species (ROS)-associated genes (sod-3, sek-1, cat-1) and daf-16 downstream genes (sod-3, ctl-2). However, it significantly increased the ROS level, malondialdehyde (MDA) content, and lipofuscin accumulation and up-regulated another daf-16 downstream gene (clk-1) (P <0.05). Interestingly, when further treated with B. longum peptide-1 (BLP-1), opposite effects were observed, and all the aforementioned indices changed significantly. In the case of RNAi (daf-16) C. elegans, BaP exposure significantly shortened the lifespan (P <0.05), which was only slightly prolonged upon further treatment with BLP-1. Furthermore, the expression of daf-16 downstream genes showed minor alterations in RNAi C. elegans upon treatment with either BaP or BLP-1. In conclusion, our findings suggest that B. longum acts as a probiotic for C. elegans. BLP-1 was shown to safeguard C. elegans from numerous oxidative damages induced by BaP, but these protective effects were contingent upon the daf-16 gene.

长双歧杆菌肽-1通过daf-16对草履虫体内苯并(α)芘诱导的氧化损伤的改善作用
氧化应激与多种疾病有关,其中苯并(α)芘(BaP)可造成严重的氧化损伤。长双歧杆菌(B. longum)被认为是某些宿主的抗氧化细菌,但它对 BaP 引发的氧化损伤的影响仍未确定。在我们的研究中,我们将不同品系的秀丽隐杆线虫(C. elegans)引入到 BaP 中以引发氧化应激,随后用不同形式的 B. longum 对其进行处理,以评估其保护作用。此外,我们还探讨了 daf-16 在这种情况下的作用。我们的研究结果表明,在野生型 N2 秀丽隐杆线虫中,长春花酵母菌--即使是以灭活菌或细菌超声裂解液(BULs)的形式--也能显著延长寿命。接触 BaP 会显著降低寿命、超氧化物歧化酶(SOD)活性和运动能力,同时下调活性氧(ROS)相关基因(sod-3、sek-1、cat-1)和 daf-16 下游基因(sod-3、ctl-2)的表达。然而,它明显增加了 ROS 水平、丙二醛(MDA)含量和脂褐素积累,并上调了另一个 daf-16 下游基因(clk-1)(P
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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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