Priority effects of forbs arriving early: the role of root interaction and asymmetric competition

Abstract

The priority effect of plant arrival is a key driver of community assembly and ecosystem succession during the restoration of degraded plant communities. However, the significance of the arrival order of different plant functional groups and their interactions with community assemblies remains unclear. Using a phytotron experiment with three fully crossed factors, we investigated the underlying mechanisms of priority effects and their relationships with the biomass and biodiversity effects in mixed plant communities by manipulating the order of arrival of species, isolation of roots, and removal of specific plants. The results showed that the strength and direction of priority effects were influenced by arrival order, root interactions, asymmetric competition among species, and their interactions. The identities of early and late-sown species also determined the magnitude of priority effects. The priority effects were stronger in grass-first (24.76%) and legume-first communities (24.48%) than in forb-first communities. The pot biomass of the different priority treatments was highest in grass-first (5.85 g), followed by legume-first (3.94 g) and forb-first (2.48 g). The order of arrival in the mixture significantly affected the net biodiversity effects (P < 0.001), which were driven by dominance effects. The community had lower overall biomass when forbs were sown first, whereas the species grown later had fewer costs with an increased overall net benefit for the resulting community. Our results emphasize that root interactions and asymmetric competition are vital determinants of order-specific priority effects in community assemblies. In addition, the importance of the priority effect of forbs sown first is related to community assembly, which may be a key determinant in successfully establishing a highly diverse community in the early stages of restoration. Species with weak competition should be considered in the early stage of community assembly. The rational use of the priority effect is conducive to improving the quality and efficiency of ecological restoration efforts.