Evaluation of the Algerbrush II rotating burr as a tool for inducing ocular surface failure in a mouse model.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2023-11-05 eCollection Date: 2023-01-01

Athar Shadmani, Trent Jarin, Xiang Qi Meng, Yugendran Rajaendran, Salih Uzun, Albert Y Wu

{"title":"Evaluation of the Algerbrush II rotating burr as a tool for inducing ocular surface failure in a mouse model.","authors":"Athar Shadmani, Trent Jarin, Xiang Qi Meng, Yugendran Rajaendran, Salih Uzun, Albert Y Wu","doi":"","DOIUrl":null,"url":null,"abstract":"Purpose: The Algerbrush II has been widely used to induce corneal and limbal injuries in animal models. The extent of injury varies with the duration of exposure, pressure from the placement of the burr, and the size of the burr. However, no study has explored the correlation between the duration of exposure and the severity of injury in mouse model with corneal and limbal stem cell deficiency (LSCD) induced using the Algerbrush II. Therefore, this study aimed to evaluate the variations in the severity of corneal and limbal injury with different durations of the Algerbrush II application.Methods: The entire cornea and limbus of C57BL/6 mice were injured for 30-45 s, 60-75 s, 90-120 s, and 3-4 min. Photography and slit-lamp examination was performed on days 0, 2, 4, and 7, followed by hematoxylin & eosin, periodic acid-Schiff, and immunohistochemical staining. Statistical analysis was performed using one way ANOVA analysis.Results: A duration of 30-45 s of injury was found to be sufficient to induce superficial corneal and limbal epithelial debridement and re-epithelialization was completed in all eyes by day 7; however, clinical signs of LSCD were not observed in all mice. Increasing the exposure time to 90-120 s resulted in central 2+ corneal opacity with limbal and paracentral corneal neovascularization. All eyes injured for 3-4 min displayed clinical signs of LSCD, such as persistent epithelial defects on day 7 after the injury, central corneal neovascularization, and 2.2+ diffuse corneal opacity. Histological signs of LSCD, including goblet cell metaplasia and K13 expression on the corneal surface, were observed in all injured eyes.Conclusions: Our findings suggest that the duration of injury is an important factor influencing the severity of LSCD in a murine model of injury. A 1-mm rotating burr was found to be more effective for keratectomy and pigment release, whereas a 0.5-mm burr was more suitable for corneal epithelial debridement.","PeriodicalId":18866,"journal":{"name":"Molecular Vision","volume":"29 ","pages":"256-265"},"PeriodicalIF":1.8000,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10784216/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Vision","FirstCategoryId":"3","ListUrlMain":"","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: The Algerbrush II has been widely used to induce corneal and limbal injuries in animal models. The extent of injury varies with the duration of exposure, pressure from the placement of the burr, and the size of the burr. However, no study has explored the correlation between the duration of exposure and the severity of injury in mouse model with corneal and limbal stem cell deficiency (LSCD) induced using the Algerbrush II. Therefore, this study aimed to evaluate the variations in the severity of corneal and limbal injury with different durations of the Algerbrush II application.

Methods: The entire cornea and limbus of C57BL/6 mice were injured for 30-45 s, 60-75 s, 90-120 s, and 3-4 min. Photography and slit-lamp examination was performed on days 0, 2, 4, and 7, followed by hematoxylin & eosin, periodic acid-Schiff, and immunohistochemical staining. Statistical analysis was performed using one way ANOVA analysis.

Results: A duration of 30-45 s of injury was found to be sufficient to induce superficial corneal and limbal epithelial debridement and re-epithelialization was completed in all eyes by day 7; however, clinical signs of LSCD were not observed in all mice. Increasing the exposure time to 90-120 s resulted in central 2+ corneal opacity with limbal and paracentral corneal neovascularization. All eyes injured for 3-4 min displayed clinical signs of LSCD, such as persistent epithelial defects on day 7 after the injury, central corneal neovascularization, and 2.2+ diffuse corneal opacity. Histological signs of LSCD, including goblet cell metaplasia and K13 expression on the corneal surface, were observed in all injured eyes.

Conclusions: Our findings suggest that the duration of injury is an important factor influencing the severity of LSCD in a murine model of injury. A 1-mm rotating burr was found to be more effective for keratectomy and pigment release, whereas a 0.5-mm burr was more suitable for corneal epithelial debridement.

本刊更多论文

将 Algerbrush II 旋转毛刺作为诱导小鼠模型眼表失败的工具进行评估。

目的：Algerbrush II 已被广泛用于诱导动物模型的角膜和角膜缘损伤。损伤的程度随暴露时间的长短、毛刺放置的压力和毛刺的大小而变化。然而，还没有研究探讨过使用阿尔杰刷 II 诱导角膜和角膜缘干细胞缺乏症（LSCD）小鼠模型的暴露持续时间与损伤严重程度之间的相关性。因此，本研究旨在评估不同的 Algerbrush II 使用时间对角膜和角膜缘损伤严重程度的影响：方法：分别在 30-45 秒、60-75 秒、90-120 秒和 3-4 分钟内损伤 C57BL/6 小鼠的整个角膜和角膜缘。在第 0、2、4 和 7 天进行照相和裂隙灯检查，然后进行苏木精和伊红、周期性酸-Schiff 和免疫组化染色。统计分析采用单因素方差分析：结果：30-45 秒的损伤时间足以诱导角膜表层和角膜缘上皮剥脱，所有小鼠的角膜表层和角膜缘上皮在第 7 天前都完成了再上皮化；但是，并非所有小鼠都出现了 LSCD 的临床症状。将暴露时间延长至 90-120 秒会导致中央 2+ 角膜翳，并伴有角膜缘和角膜旁新生血管。所有受伤 3-4 分钟的眼睛都显示出 LSCD 的临床症状，如受伤后第 7 天出现持续性上皮缺损、角膜中央新生血管和 2.2+ 弥漫性角膜混浊。在所有受伤的眼睛中都观察到了 LSCD 的组织学迹象，包括角膜表面的鹅口疮细胞增生和 K13 表达：我们的研究结果表明，在小鼠损伤模型中，损伤持续时间是影响 LSCD 严重程度的重要因素。我们发现，1 毫米的旋转锉刀对角膜切除和色素释放更有效，而 0.5 毫米的锉刀更适合角膜上皮清创。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.