Effects of co-inoculation of indole-3-acetic acid- and ammonia-producing bacteria on plant growth and nutrition, soil elements, and the relationships of soil microbiomes with soil physicochemical parameters

IF 1.8 Q2 AGRICULTURE, MULTIDISCIPLINARY
N. Pongsilp, P. Nimnoi
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Abstract

Abstract To cope with the problem of the reduction in agricultural productivity, the massive usage of chemical fertilizers for boosting agricultural productivity is popular across the world. In return, it thereby declines soil quality and biodiversity. We therefore determined the potential of co-inoculation of the indole-3-acetic-acid- and ammonia-producing bacteria, Pseudomonas sp. strain NK2/7 and Bacillus sp. strain NK1/19, immobilized in agar to promote mustard greens growth and investigated the effects of co-inoculation of both strains on plant and soil elements as well as soil bacterial community structure, in comparison with a chemical fertilizer and single-strain inoculations. The knowledge on the relationships of both species with plant and soil microbiota in field conditions is limited. The results show that the co-inoculation of both strains immobilized in agar increased plant dry weight up to 62.02% and plant elements, including N (38.46%), P (28.37%), K (203.16%), Ca (10.89%), Mg (25.8%), and Na (70.3%). The co-inoculation also conferred the highest organic matter (OM) (by 429.45%) and increased soil elements including Fe (92.74%), Mn (126.88%), Cu (12.05%), and Zn (107.46%). Illumina next-generation sequencing demonstrated that the establishment of both strains affected the soil bacterial community by reducing bacterial diversity and richness. Bacterial classes Bacteroidia, alpha-Proteobacteria, Vicinamibacteria, Gemmatimonadetes, Verrucomicrobiae, Polyangia, Nitrososphaeria, and Blastocatellia were significantly decreased, whereas the amounts of gamma-Proteobacteria and Bacilli were significantly increased. Soil parameters, including pH, OM, total Fe, Mn, Cu, and Zn, were the major factors influencing the soil bacterial community structure.
共同接种吲哚-3-乙酸和产氨菌对植物生长和营养、土壤元素的影响,以及土壤微生物群与土壤理化参数的关系
摘要 为应对农业生产率下降的问题,大量使用化肥来提高农业生产率的做法在全世界都很流行。然而,化肥的使用却导致土壤质量和生物多样性下降。因此,我们测定了固定在琼脂中的产吲哚-3-乙酸和氨的假单胞菌菌株 NK2/7 和芽孢杆菌菌株 NK1/19 共同接种促进芥菜生长的潜力,并研究了与化肥和单一菌株接种相比,两种菌株共同接种对植物和土壤元素以及土壤细菌群落结构的影响。在田间条件下,关于这两种菌株与植物和土壤微生物群的关系的知识十分有限。结果表明,固定在琼脂中的两种菌株共同接种后,植物干重增加了 62.02%,植物元素包括氮(38.46%)、磷(28.37%)、钾(203.16%)、钙(10.89%)、镁(25.8%)和钠(70.3%)。共同接种还产生了最高的有机质(OM)(429.45%),并增加了土壤元素,包括铁(92.74%)、锰(126.88%)、铜(12.05%)和锌(107.46%)。Illumina 下一代测序表明,这两种菌株的建立影响了土壤细菌群落,降低了细菌的多样性和丰富度。类杆菌属、α-蛋白菌属、维西那米菌属、宝石藻菌属、疣藻菌属、多孔菌属、硝化细菌属和高疫病菌属的数量显著减少,而γ-蛋白菌属和芽孢杆菌属的数量显著增加。土壤参数(包括 pH 值、OM 值、总铁、锰、铜和锌)是影响土壤细菌群落结构的主要因素。
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来源期刊
Open Agriculture
Open Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
3.80
自引率
4.30%
发文量
61
审稿时长
9 weeks
期刊介绍: Open Agriculture is an open access journal that publishes original articles reflecting the latest achievements on agro-ecology, soil science, plant science, horticulture, forestry, wood technology, zootechnics and veterinary medicine, entomology, aquaculture, hydrology, food science, agricultural economics, agricultural engineering, climate-based agriculture, amelioration, social sciences in agriculuture, smart farming technologies, farm management.
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