Genetic Polymorphism of Growth Hormone Gene in Dorper Sheep using PCR-RFLP

IF 0.4 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Research Pub Date : 2024-01-02 DOI:10.18805/ijar.bf-1683

Molabe Kagisho Madikadike, Thobela Louis Tyasi, Vusi Mbazima, B. Gunya

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引用次数: 0

Abstract

Background: The growth hormone gene has a significant impact on postnatal longitudinal growth and development as well as the metabolism of protein, carbohydrates, and fats moreover its effects indirectly relate to bone and skeletal development. The objective of the study was to identify a single nucleotide polymorphism of the growth hormone gene and its association with growth traits in Dorper sheep. Methods: Data were collected from 50 Dorper sheep of 1 to 2 years of age at the University of Limpopo Experimental farm. The objective of the study was achieved by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and General Linear Model (GLM). Result: PCR-RFLP reports revealed different segment patterns resulting in two genotypes such as AA and AB genotypes. Marker-traits association analysis showed that no significant difference was observed between AA and AB genotypes regarding all the measured traits except with wither height and AA genotype was remarkably related to higher withers height than AB genotype. It can be concluded that GH1 could be used as a genetic marker for marker-assisted selection (MAS) during breeding for the improvement of withers height.

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利用 PCR-RFLP 测定多尔巴绵羊生长激素基因的遗传多态性

背景：生长激素基因对绵羊出生后的纵向生长发育以及蛋白质、碳水化合物和脂肪的新陈代谢有重要影响，而且其影响还间接关系到骨骼和骨骼发育。本研究旨在确定生长激素基因的单核苷酸多态性及其与多尔巴绵羊生长性状的关系。研究方法从林波波大学实验农场 50 只 1 至 2 岁的多尔帕绵羊中收集数据。通过聚合酶链式反应-限制性片段长度多态性和一般线性模型（GLM）达到研究目的。结果：PCR-RFLP 报告显示，不同的片段模式会产生两种基因型，如 AA 和 AB 基因型。标记-性状关联分析表明，AA 基因型和 AB 基因型在所有测定性状上均无显着差异，只有在干高度上，AA 基因型比 AB 基因型与更高的干高度明显相关。由此可以得出结论，GH1 可作为育种过程中标记辅助选择（MAS）的遗传标记，用于改善腰高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Animal Research AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.00

自引率

20.00%

发文量

332

审稿时长

6 months

期刊介绍： The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.