Phytochemical Content, Anti-inflammatory, Anti-apoptotic, and Antioxidant Activities of Dwarf Elder (Sambucus Ebulus) Against Nasal Polyposis

IF 1 Q4 PHARMACOLOGY & PHARMACY
Babak Pourgholamali, Fatemeh Yosefbeyk, Masoud Ansar, Arash Zaminy, Shadman Nemati, Sina Ramezani, Hamidreza Bagheri, M. Faghani
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Abstract

Background: Sambucus Ebulus L. (SE) is known as an anti-inflammatory herb in traditional medicine. Nasosinusal polyposis is a common type of chronic nose and paranasal sinus inflammation. It is more common in patients with asthma and aspirin-exacerbated respiratory diseases. Objectives: This study aimed to investigate the apoptotic and anti-inflammatory effects of SE fruit extract on NP. Methods: The extract of SE fruit was prepared and subjected to total phenolic, anthocyanin, and flavonoid content measurement. The antioxidant activity was tested using the DPPH radical scavenging method. Nasal polyp (NP) tissue samples were collected from patients. Different concentrations of the SE extract were exposed to NPT samples for 24 hours. The expression of BAX and BAD proapoptotic markers, IL-5 and GM-CSF levels, and cell apoptosis were evaluated by real-time PCR, ELISA, and TUNEL assay, respectively. Results: The total phenolic and flavonoid contents of the extract were 38.44 (mg GAE/g extract) and 8.62 ± 0.12 (mg QE/g extract), respectively. Moreover, the total anthocyanin content was 0.56 ± 0.01 (mg C3GE/g extract). The IC50 of SE fruit extract in the DPPH radical scavenging assay was 190.78 ± 0.55 µg/mL. Also, BAX and BAD markers and TUNEL-positive cells were observed to increase in NP tissue samples in vitro after treatment with SE fruit extract (P < 0.05). The level of GM-CSF in the treated groups was reduced (P < 0.05). Conclusions: Our results showed that SE fruit extract was a good source of phenolic compounds that can induce anti-inflammatory and anti-apoptotic events in NP tissues, at least partly through increasing the expression of BAD and BAX apoptotic markers and reducing GM-CSF levels. The clinical applicability of SE fruit extract in the treatment of nasal polyps should be investigated in the future.
矮接骨木(Sambucus Ebulus)的植物化学成分含量、抗炎、抗凋亡和抗氧化活性对鼻息肉病的作用
背景:Sambucus Ebulus L.(SE)在传统医学中被称为消炎草药。鼻窦息肉病是一种常见的慢性鼻和副鼻窦炎。它在哮喘和阿司匹林加重的呼吸道疾病患者中更为常见。研究目的本研究旨在探讨 SE 果实提取物对鼻腔息肉的凋亡和抗炎作用。方法:制备 SE 果实提取物并测定其总酚、花青素和类黄酮含量。采用 DPPH 自由基清除法测试其抗氧化活性。从患者身上采集鼻息肉(NP)组织样本。将不同浓度的 SE 提取物与 NPT 样品接触 24 小时。通过实时 PCR、ELISA 和 TUNEL 检测法分别评估了 BAX 和 BAD 促凋亡标志物的表达、IL-5 和 GM-CSF 水平以及细胞凋亡情况。结果提取物的总酚和类黄酮含量分别为 38.44(毫克 GAE/克提取物)和 8.62 ± 0.12(毫克 QE/克提取物)。此外,花青素总含量为 0.56 ± 0.01(毫克 C3GE/克提取物)。在 DPPH 自由基清除试验中,SE 果实提取物的 IC50 为 190.78 ± 0.55 µg/mL。此外,经 SE 果实提取物处理后,体外观察到 NP 组织样本中的 BAX 和 BAD 标记以及 TUNEL 阳性细胞有所增加(P < 0.05)。治疗组的 GM-CSF 水平降低(P < 0.05)。结论我们的研究结果表明,SE 果实提取物是一种良好的酚类化合物来源,可诱导 NP 组织中的抗炎和抗凋亡事件,至少部分是通过增加 BAD 和 BAX 凋亡标记物的表达以及降低 GM-CSF 水平来实现的。未来应研究 SE 果实提取物在治疗鼻息肉方面的临床适用性。
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CiteScore
1.40
自引率
0.00%
发文量
26
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