Determination of Effect of Supplementation of Oak Tannin Extract on Digestibility, Metabolisable Energy, Methane Production and Ammonia Production of Lamb Diets

Ahmet Salih Demi̇r, Adem Kamalak
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Abstract

The aim of current experiment was to determine the effect of supplementation of oak tannin extract on gas production, methane production, digestibility, metabolisable energy, and ammonia production of lamb diets using in vitro gas production technique. Oak tannin extract was included into total mixed ration at the 0, 2, 4 and 6 % on a dry matter basis. Although supplementation of oak tannin had no significant effect on gas, methane whereas supplementation had a significant effect on ammonia production of lamb diets. Gas and methane production of total mixed rations ranged from 50.25 to 53.25 ml and 7.72 to 8.15 ml respectively. Ammonia concentration of mixed rations ranged from 54.97 to 62.67 mg/100 ml. The decrease in ammonia of lamb diets per g oak supplementation was 0.1263 mg /100 mL. Metabolisable energy and organic matter digestibility of lamb diets ranged from 10.42 to 10.80 MJ kg DM and 70.27 to 73.02 % respectively. This study clearly showed that oak tannin had an anti-proteolytic potential for ruminant animals and supplementation of oak tannin significantly reduced ammonia production without compromising digestibility of diets. Therefore oak tannin can be used to manipulate the rate and extent of degradation of protein in the rumen. However before large implication, oak tannin should be further investigated using in vivo experiment to determine the toxic level of oak tannin in ruminant animals.
测定添加橡树单宁提取物对羔羊日粮消化率、代谢能、甲烷产量和氨产量的影响
本实验的目的是利用体外产气技术确定添加橡树单宁提取物对羔羊日粮产气量、甲烷产量、消化率、代谢能和氨产量的影响。在混合日粮中添加的橡树单宁提取物的干物质含量分别为 0%、2%、4% 和 6%。虽然添加橡树单宁对羔羊日粮的气体和甲烷产生量没有显著影响,但对羔羊日粮的氨产生量有显著影响。总混合日粮的气体和甲烷产量分别为 50.25 至 53.25 毫升和 7.72 至 8.15 毫升。混合日粮的氨浓度范围为 54.97 至 62.67 毫克/100 毫升。每添加 1 克橡木,羔羊日粮中的氨减少 0.1263 毫克/100 毫升。羔羊日粮的代谢能和有机物消化率分别为 10.42 至 10.80 MJ kg DM 和 70.27 至 73.02 %。这项研究清楚地表明,橡树单宁对反刍动物具有抗蛋白质分解的潜力,在不影响日粮消化率的情况下,补充橡树单宁可显著减少氨的产生。因此,橡木单宁可用于控制蛋白质在瘤胃中的降解速度和程度。不过,在进行大规模应用之前,应通过体内实验进一步研究橡树单宁,以确定橡树单宁在反刍动物体内的毒性水平。
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