Characterization of the histological changes in ovaries of Goto-Kakizaki diabetic rats

IF 0.3 Q4 ANATOMY & MORPHOLOGY

European journal of anatomy Pub Date : 2024-01-09 DOI:10.52083/sjtl7284

Cristian Montero-Peña, José M. Morales-De Pando, Alfredo Díaz-Gómez, Gloria González-Medina, A. Ribelles-García, Gonzalo Pérez-Arana, J. Prada-Oliveira

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Abstract

Goto-Kakizaki (GK) rats are a useful animal model for studying type 2 diabetes mellitus (T2DM). However, this strain of rats exhibits poor fertility, so it is difficult to expand colonies. Based on previous studies, it was hypothesized that alterations in the oestrous cycle of diabetic rats are related to ovarian histology. The aim of this study was to determine the histology of the ovaries of diabetic rats compared to nondiabetic rats to understand the poor fertility of this strain of diabetic rats. An experimental study was thus conducted. Eight GK rats and eight Wistar rats were utilized. The rats were age-adjusted into two groups called “young rats” and “mature rats” at two and fourteen months of age, respectively. After sacrificing the rats, the ovaries were dissected and processed by fixation and paraffin embedding to perform the histological study. The results included the number of corpora lutea and the percentage of follicular fraction in each ovary, as well as qualitative data such as the presence of follicles in different stages of development. Our findings revealed differences between GK and Wistar rats. The ovarian histological findings were related to the presence of T2DM, polycystic ovary syndrome and poor fertility in female GK rats; the link between these pathologies is insulin resistance. Future lines of investigation into metabolic treatment, which may help improve insulin resistance, could also benefit the previously described pathologies in female GK rats.

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后藤柿崎糖尿病大鼠卵巢组织学变化的特征

后藤柿崎（GK）大鼠是研究 2 型糖尿病（T2DM）的有用动物模型。然而，该品系大鼠的繁殖能力较差，因此很难扩大群体。根据以往的研究，我们推测糖尿病大鼠发情周期的改变与卵巢组织学有关。本研究的目的是测定糖尿病大鼠与非糖尿病大鼠卵巢组织学的比较，以了解该品系糖尿病大鼠生育能力低下的原因。因此进行了一项实验研究。实验中使用了 8 只 GK 大鼠和 8 只 Wistar 大鼠。大鼠在两个月龄和十四个月龄时分别被分为两组，称为 "幼鼠 "和 "成熟鼠"。将大鼠处死后，解剖卵巢并进行固定和石蜡包埋处理，以进行组织学研究。结果包括每个卵巢中黄体的数量和卵泡部分的百分比，以及定性数据，如不同发育阶段卵泡的存在情况。我们的研究结果表明，GK 大鼠和 Wistar 大鼠之间存在差异。卵巢组织学发现与雌性 GK 大鼠的 T2DM、多囊卵巢综合征和生育能力低下有关；这些病症之间的联系是胰岛素抵抗。未来对代谢治疗的研究方向可能有助于改善胰岛素抵抗，也会对之前描述的雌性 GK 大鼠的病理变化有益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European journal of anatomy ANATOMY & MORPHOLOGY-

CiteScore

0.60

自引率

33.30%

发文量

期刊介绍： El European Journal of Anatomy es continuación de la revista “Anales de Anatomía”, publicada en español desde 1952 a 1993. Tras unos años de interrupción debido fundamentalmente a problemas económicos para su mantenimiento, la Sociedad Anatómica Española quiso dar un nuevo impulso a dicha publicación, por lo que fue sustituido su título por el actual, además de ser publicada íntegramente en inglés para procurar así una mayor difusión fuera de nuestras fronteras. Este nuevo periodo se inició en 1996 completándose el primer volumen durante el año 1997.