Exhaled breath is found to be better than blood samples for determining propofol concentrations in the brain tissues of rats.

IF 3.7 4区医学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of breath research Pub Date : 2024-01-24 DOI:10.1088/1752-7163/ad1d65

Xiaoxiao Li, Pan Chang, Xing Liu, Yi Kang, Zhongjun Zhao, Yixiang Duan, Jin Liu, Wensheng Zhang

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引用次数: 0

Abstract

The correlation between propofol concentration in exhaled breath (C_E) and plasma (C_P) has been well-established, but its applicability for estimating the concentration in brain tissues (C_B) remains unknown. Given the impracticality of directly sampling human brain tissues, rats are commonly used as a pharmacokinetic model due to their similar drug-metabolizing processes to humans. In this study, we measuredC_E,C_P, andC_Bin mechanically ventilated rats injected with propofol. Exhaled breath samples from the rats were collected every 20 s and analyzed using our team's developed vacuum ultraviolet time-of-flight mass spectrometry. Additionally, femoral artery blood samples and brain tissue samples at different time points were collected and measured using high-performance liquid chromatography mass spectrometry. The results demonstrated that propofol concentration in exhaled breath exhibited stronger correlations with that in brain tissues compared to plasma levels, suggesting its potential suitability for reflecting anesthetic action sites' concentrations and anesthesia titration. Our study provides valuable animal data supporting future clinical applications.

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在测定大鼠脑组织中的异丙酚浓度时，发现呼气比血液样本更好。

丙泊酚在呼出气体（CE）和血浆（CP）中的浓度之间的相关性已得到证实，但其是否适用于估计脑组织（CB）中的浓度仍是未知数。由于直接采集人类脑组织样本并不现实，大鼠的药物代谢过程与人类相似，因此通常被用作药代动力学模型。在本研究中，我们测量了注射异丙酚的机械通气大鼠的 CE、CP 和 CB。每隔 20 秒收集一次大鼠的呼气样本，并使用我们团队开发的真空紫外飞行时间质谱（VUV TOF-MS）进行分析。此外，还收集了不同时间点的股动脉血样本和脑组织样本，并使用高效液相色谱质谱法进行了测定。结果表明，与血浆水平相比，呼出气体中的异丙酚浓度与脑组织中的异丙酚浓度具有更强的相关性，这表明异丙酚可能适用于反映麻醉作用部位的浓度和麻醉滴定。我们的研究为未来的临床应用提供了宝贵的动物数据支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM

CiteScore

7.60

自引率

21.10%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.