Nicotinamide employs a starvation strategy against Porphyromonas gingivalis virulence by inhibiting the heme uptake system and gingipain activities.

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2024-10-01 Epub Date: 2024-01-10 DOI:10.1111/omi.12448
Zixue Lei, Qizhao Ma, Yeting Tu, Yang Qiu, Tao Gong, Yongwang Lin, Xuedong Zhou, Yuqing Li
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引用次数: 0

Abstract

Periodontitis is a common oral bacterial infection characterized by inflammatory responses. Its high prevalence lowers the quality of life for individuals and increases the global economic and disease burden. As microorganisms in dental plaque are responsible for this oral disease, antibacterial drug treatments are effective strategies for preventing and treating periodontitis. In this study, we investigated the inhibitory effect of nicotinamide (NAM), a vitamin B3 derivative, on the growth and virulence of Porphyromonas gingivalis, a key member of the red complex. Our findings revealed that NAM inhibited bacterial growth and gingipain activities, which played a dominant role in protein hydrolysis and heme acquisition. NAM decreased hemagglutination and hemolysis abilities and changed hemin and hemoglobin binding capacities, controlling bacterial infection through a starvation strategy by blocking access to growth-essential nutrients from the outside and reducing bacterial virulence. Several experiments in an animal model showed the effectiveness of NAM in preventing alveolar bone loss and reducing inflammatory cell infiltration, shedding light on its potential therapeutic applicability.

烟酰胺通过抑制血红素摄取系统和gingipain活性,对牙龈卟啉单胞菌的毒力采取饥饿策略。
牙周炎是一种以炎症反应为特征的常见口腔细菌感染。它的高发病率降低了个人的生活质量,增加了全球的经济和疾病负担。牙菌斑中的微生物是导致这种口腔疾病的罪魁祸首,因此抗菌药物治疗是预防和治疗牙周炎的有效策略。在这项研究中,我们研究了维生素 B3 衍生物烟酰胺(NAM)对牙龈卟啉单胞菌(红色复合菌群的主要成员)的生长和毒力的抑制作用。我们的研究结果表明,NAM 可抑制细菌的生长和gingipain 活性,而gingipain 在蛋白质水解和血红素获取过程中起着主导作用。NAM 降低了血凝和溶血能力,改变了血红素和血红蛋白的结合能力,通过饥饿策略控制细菌感染,阻止细菌从外部获得生长所需的营养物质,降低细菌的毒力。在动物模型中进行的几项实验表明,NAM 在防止牙槽骨流失和减少炎症细胞浸润方面非常有效,从而揭示了其潜在的治疗适用性。
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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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