Establishing Single-Cell Clones from In Vitro-Cultured Circulating Tumor Cells.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3621-3_8

Teng Teng, Min Yu

引用次数: 0

Abstract

Cancer is a common health problem with more than 90% of deaths due to metastases. Circulating tumor cells (CTCs) contain precursors that can initiate metastases. However, CTCs are rare, heterogeneous, and difficult to expand in culture. We have previously created CTC-derived cell lines from stage IV breast cancer patients. These CTC lines were used to establish single-cell CTC clones using flow cytometry cell sorting.

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从体外培养的循环肿瘤细胞中建立单细胞克隆。

癌症是一种常见的健康问题，90% 以上的死亡都是由于癌症转移造成的。循环肿瘤细胞（CTC）含有可引发转移的前体。然而，CTCs 是稀有的、异质性的，而且很难在培养中扩增。我们以前曾从 IV 期乳腺癌患者身上提取了 CTC 衍生细胞系。利用流式细胞仪细胞分拣技术，这些 CTC 系被用来建立单细胞 CTC 克隆。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.

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