A molecular and bacteriological survey of Brucella spp. in wild venomous snake in Iran

Abstract

Brucellosis is a neglected zoonotic disease with significant public health impacts. It is primarily associated with classic Brucella species in mammals, but recent research has shown atypical Brucella strains in various hosts, including reptiles. Despite the known physiological differences between reptiles and humans, the rise in invasive reptile populations and other factors make reptiles and the pathogens they carry a potential public health concern. This study collected 90 venomous snakes from different regions in Iran and examined their liver samples, buccal, and cloaca cavities for Brucella spp. The samples underwent bacteriological analysis and DNA extraction. PCR amplification of the Omp28 gene and IS711-based PCR were used for the molecular identification of Brucella species. Subsequently, the Omp28 gene sequences were determined, and a phylogenetic tree was created. Brucella abortus was detected in the liver tissue of two snake species, namely Pseudocerastes persicus and Vipera albicornuta, at a prevalence rate of 2.2%. This finding is exceptional, as it has not been previously documented in venomous snake populations in the wild. The study confirms the presence of Brucella spp. in venomous snakes, suggesting the potential for zoonotic transmission. This discovery raises questions about the evolutionary forces shaping Brucella populations in reptiles. Understanding the prevalence and impact of Brucella in snakes is essential for wildlife conservation and assessing zoonotic risks. Proper safety measures are crucial when handling reptiles that may carry Brucella. Further research is needed to fill knowledge gaps regarding Brucella in these snake species and its potential zoonotic implications.