Cinnamaldehyde Acts as a Fungistat by Disrupting the Integrity of Fusarium oxysporum Fox-1 Cell Membranes

IF 3.1 3区 农林科学 Q1 HORTICULTURE
Li-Rong Zhou, Hai-Jun Hu, Jie Wang, Yong-Xing Zhu, Xue-Dong Zhu, Jia-Wei Ma, Yi-Qing Liu
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Abstract

To counter the harmful impacts of agricultural chemicals on the environment and human health, there is an increasing demand for safe, eco-friendly, and potent plant-based biopesticides. In this study, we aimed to investigate the antimicrobial effects of ginger essential oil and selected volatile compounds (linalool, eugenol, citral, and cinnamaldehyde [CA]) against Fusarium oxysporum FOX-1. Minimum inhibitory concentrations (MICs) were determined using the mycelium growth inhibition method. The compound CA exhibited the most potent antifungal effect against F. oxysporum FOX-1 and was selected for further investigation. After treatment with CA at 1/2 MIC or MIC, the spore germination of F. oxysporum FOX-1 was significantly inhibited at 12 h. Furthermore, microscopic observation revealed that CA treatment resulted in the morphological degradation of F. oxysporum FOX-1. CA destroyed the cell membrane integrity of F. oxysporum FOX-1, increasing the relative conductivity and the leakage of intracellular protein, nucleic acids, and malondialdehyde, affecting the integrity and metabolism of the cell membrane. The effects were positively related to CA concentration. Additionally, in vivo experiments with rhizome sections showed that CA significantly reduced the pathogenicity of F. oxysporum FOX-1. Overall, these findings provide evidence for the potential of using ginger essential oil components as fungicides, offering a basis for future research to develop robust and eco-friendly plant-derived fungicides that serve as a sustainable means to reduce fungus-driven agricultural losses.
肉桂醛通过破坏 Fusarium oxysporum Fox-1 细胞膜的完整性发挥杀真菌剂的作用
为了应对农用化学品对环境和人类健康的有害影响,人们对安全、环保、高效的植物性生物农药的需求与日俱增。在这项研究中,我们旨在调查生姜精油和选定挥发性化合物(芳樟醇、丁香酚、柠檬醛和肉桂醛 [CA])对 Fusarium oxysporum FOX-1 的抗菌效果。采用菌丝生长抑制法测定了最低抑菌浓度(MIC)。化合物 CA 对 F. oxysporum FOX-1 的抗真菌效果最强,因此被选为进一步研究的对象。用浓度为 1/2 MIC 或 MIC 的 CA 处理 F. oxysporum FOX-1 后,12 h 后其孢子萌发明显受到抑制。CA 破坏了 F. oxysporum FOX-1 细胞膜的完整性,增加了相对电导率和细胞内蛋白质、核酸及丙二醛的渗漏,影响了细胞膜的完整性和新陈代谢。这些影响与 CA 浓度呈正相关。此外,根茎切片的体内实验表明,CA 能显著降低 F. oxysporum FOX-1 的致病性。总之,这些发现为利用生姜精油成分作为杀菌剂的潜力提供了证据,为未来研究开发稳健、生态友好的植物源杀菌剂提供了基础,可作为减少真菌导致的农业损失的可持续手段。
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来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
期刊介绍: Horticulturae (ISSN 2311-7524) is an international, multidisciplinary, peer-reviewed, open access journal focusing on all areas and aspects of temperate to tropical horticulture. It publishes original empirical and theoretical research articles, short communications, reviews, and opinion articles. We intend to encourage scientists to publish and communicate their results concerning all branches of horticulture in a timely manner and in an open venue, after being evaluated by the journal editors and randomly selected independent expert reviewers, so that all articles will never be judged in relation to how much they confirm or criticize the opinions of other researchers.
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